Molecular characterization of Psidium guajava genotypes using microsatellite markers
摘要
A set of twenty-four Psidium guajava genotypes including exotic cultivar had been genetically characterized using a total of twenty microsatellite (SSR) markers spanning a total of 11 chromosomes. The average number of alleles per locus was 2.93. Fifteen markers (out of the twenty used) were highly polymorphic and could discriminate local cultivars and exotic collection. Two markers, mPgCIR352 and mPgCIR175, showed the highest level of polymorphism, whereas marker mPgCIR352 showed the maximum marker index for high level of resolving capacity amongst all markers tested. Marker mPgCIR321, with 0.778 PIC value present on linkage group 11, has been identified to be associated with fruit yield. Molecular variance study resulted at 92% genetic variance within population and 8% between population. First three axes explained 53.32% variation through principal coordinate analysis. This study explains variation among the existing popular cultivar, local cultivar, and few exotic cultivars which can be helpful in designing cross breeding programmes through the selection of the superior parental varieties.