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<p>This study aims to determine the yield, and the total content of phenols and flavonoids as well as to evaluate the antioxidant activity of the decoction extract (DE) and three hydroethanolic extracts obtained by reflux heating (RE), maceration (ME) and ultrasound (UE) methods of Anvillea garcinii subsp. radiata. The antioxidant activity was assessed using spectrophotometric assays 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3) acid ethylbenzothiazoline-6-sulfonic acid (ABTS) and electrochemical cyclic voltammetry (CV) measurements. The results show that hydroethanolic extracts are rich in polyphenols and flavonoids and have better antioxidant activity than aqueous DE. The ME and UE showed interesting antioxidant activity, with respective IC<sub>50</sub> values of 34.08 ± 0.92 and 43.39 ± 1.11&#xa0;µg/ml for DPPH, and 21.50 ± 0.34 and 23.85 ± 0.14&#xa0;µg/ml in ABTS assay. The RE recorded an IC50 of 53.97 ± 41 and 29.24 ± 0.48&#xa0;µg/ml in the DPPH and ABTS assay, respectively. While the DE showed relatively low antioxidant activity, with an IC50 of 84.57 ± 0.58&#xa0;µg/ml in the DPPH test. For total antioxidant capacity, CV test reveals that the ME, UE and RE extracts recorded interesting values of 40.68 ± 0.53, 32.79 ± 0.96 and 24.06 ± 0.59 mgAAE/g, respectively, while the DE value was around 11.63 ± 0.37 mgAAE/g.</p>

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Effect of extraction methods on yield, total polyphenols and flavonoids contents and antioxidant activity of Anvillea garcinii subsp. radiata using spectrophotometric and electrochemical approaches

  • 期刊论文

  • 发表日期：

    2025年4月25日

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  • Lahcen Oucheikh,
  • Youssef Youssefi,
  • Omar Ou-Ani,
  • Abdelaziz Ait Sidi Mou,
  • Mohamed Znini,
  • Ahmad Oubair,
  • Elhoussine Mabrouk,
  • Driss Chebabe

摘要

This study aims to determine the yield, and the total content of phenols and flavonoids as well as to evaluate the antioxidant activity of the decoction extract (DE) and three hydroethanolic extracts obtained by reflux heating (RE), maceration (ME) and ultrasound (UE) methods of Anvillea garcinii subsp. radiata. The antioxidant activity was assessed using spectrophotometric assays 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3) acid ethylbenzothiazoline-6-sulfonic acid (ABTS) and electrochemical cyclic voltammetry (CV) measurements. The results show that hydroethanolic extracts are rich in polyphenols and flavonoids and have better antioxidant activity than aqueous DE. The ME and UE showed interesting antioxidant activity, with respective IC50 values of 34.08 ± 0.92 and 43.39 ± 1.11 µg/ml for DPPH, and 21.50 ± 0.34 and 23.85 ± 0.14 µg/ml in ABTS assay. The RE recorded an IC50 of 53.97 ± 41 and 29.24 ± 0.48 µg/ml in the DPPH and ABTS assay, respectively. While the DE showed relatively low antioxidant activity, with an IC50 of 84.57 ± 0.58 µg/ml in the DPPH test. For total antioxidant capacity, CV test reveals that the ME, UE and RE extracts recorded interesting values of 40.68 ± 0.53, 32.79 ± 0.96 and 24.06 ± 0.59 mgAAE/g, respectively, while the DE value was around 11.63 ± 0.37 mgAAE/g.