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<p><i>Pfaffia glomerata</i>, commonly known as Brazilian-ginseng, attracts significant pharmaceutical interest due to its β-ecdysone content. However, the extraction of phytotherapeutic agents can threaten the existence of medicinal plants and contribute to their genetic erosion. Cryopreservation is the most promising technique for the long-term maintenance of plant genetic resources. This study aimed to establish a cryopreservation method for different accessions of <i>P. glomerata</i>, analyzing the impact of pre-cultivation, saturation, and vitrification solutions. Optimal cryopreservation involved pre-cultivation with 0.75&#xa0;M sucrose at 4&#xa0;°C under constant agitation for 24&#xa0;h, followed by incubation in 2.0&#xa0;M glycerol and 0.4&#xa0;M sucrose for 20&#xa0;min, and immersion in a plant vitrification solution (comprising 30.0% glycerol, 15.0% ethylene glycol, 15.0% dimethyl sulfoxide, and 13.6% sucrose) for 1&#xa0;h. This method enabled the survival and regeneration of explants after exposure to liquid nitrogen. Next, the impact of photoautotrophic and photomixotrophic cultivation on the hardening of explants at ultra-low temperatures was assessed. Photoautotrophic conditions increased the proportion of vacuoles and limited nuclear development; whereas photomixotrophic cultivation of four <i>P. glomerata</i> accessions supplemented with 4.0 or 40.0 μM abscisic acid and 10.0 or 100.0 μM salicylic acid was more successful. Pre-acclimatization was found to be more efficient with 40 μM abscisic acid, and survival was greater in accessions with a higher accumulation of β-ecdysone.</p>

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Cryopreservation and photomixotrophic cultivation of Pfaffia glomerata (Spreng.) pedersen genotypes enable the conservation of a valuable medicinal resource

  • 期刊论文

  • 发表日期：

    2025年3月7日

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  • Duanny Thais Rodrigues Caproni,
  • Gabriela Torres-Silva,
  • Diego Silva Batista,
  • Ana Cláudia Ferreira Cruz,
  • Sérgio Heitor Sousa Felipe,
  • Kristhiano Chagas,
  • Marcos Vinícius Marques Pinheiro,
  • Wagner Campos Otoni

摘要

Pfaffia glomerata, commonly known as Brazilian-ginseng, attracts significant pharmaceutical interest due to its β-ecdysone content. However, the extraction of phytotherapeutic agents can threaten the existence of medicinal plants and contribute to their genetic erosion. Cryopreservation is the most promising technique for the long-term maintenance of plant genetic resources. This study aimed to establish a cryopreservation method for different accessions of P. glomerata, analyzing the impact of pre-cultivation, saturation, and vitrification solutions. Optimal cryopreservation involved pre-cultivation with 0.75 M sucrose at 4 °C under constant agitation for 24 h, followed by incubation in 2.0 M glycerol and 0.4 M sucrose for 20 min, and immersion in a plant vitrification solution (comprising 30.0% glycerol, 15.0% ethylene glycol, 15.0% dimethyl sulfoxide, and 13.6% sucrose) for 1 h. This method enabled the survival and regeneration of explants after exposure to liquid nitrogen. Next, the impact of photoautotrophic and photomixotrophic cultivation on the hardening of explants at ultra-low temperatures was assessed. Photoautotrophic conditions increased the proportion of vacuoles and limited nuclear development; whereas photomixotrophic cultivation of four P. glomerata accessions supplemented with 4.0 or 40.0 μM abscisic acid and 10.0 or 100.0 μM salicylic acid was more successful. Pre-acclimatization was found to be more efficient with 40 μM abscisic acid, and survival was greater in accessions with a higher accumulation of β-ecdysone.