<p>Potato (<i>Solanum tuberosum</i> L.) is a perennial food crop that helps to ensure global food and nutrition security. This study developed an in vitro callus and shoot induction protocol for the potato cultivar Kufri Sangam and assessed the frequency of callus and shoot regeneration. This study found that supplementing the Murashige and Skoog&#xa0;(MS) medium with 0.5 mgL⁻¹ 6-Benzylaminopurine (BAP) and 5 mgL⁻¹ 1-Naphthaleneacetic acid (NAA) resulted in 100% callus induction whereas 2 mgL⁻¹ thiadiazuron (TDZ) and 0.25 mgL⁻¹ 1-Naphthaleneacetic acid (NAA) was most effective for shoot induction. For shoot proliferation, MS media supplemented with zeatin riboside (1 mgL⁻¹), NAA (0.5 mgL⁻¹), and GA₃ (5 mgL⁻¹), resulted in 73.33% shoot regeneration. The effect of varying indole butyric acid (IBA) on root induction showed the best results when MS medium was supplemented with 1.0 mgL⁻¹&#xa0;IBA. By allowing the micropropagation of an isogenic population in a highly heterozygous potato plant, well-optimized culture conditions and the right combinations of growth regulators guarantee an effective regeneration technique for a particular potato cultivar and reduce the likelihood of somaclonal variants. The application of biotechnological developments in gene transfer and ploidy level maintenance in the potato cultivar Kufri Sangam may benefit from the deployment of this innovative approach.</p>