<p>Cavitation is a transient and dynamic phenomenon that refers to the formation of bubbles in fluid when negative pressure in a fluid is dropped below its vapor pressure. Recently, cavitation in soft materials has gained attention due to its relevance in both therapeutic diagnostics and potential source of brain injury. In general, cavitation can occur in soft materials with homogenous or heterogenous microstructure. While cavitation in homogeneous materials is relatively well-studied, our understanding of cavitation dynamics in heterogeneous media, such as biological tissues and engineered materials, is limited. In particular, the role of interfaces on cavitation dynamics is not well-understood. Using a custom-designed drop-mass impactor system integrated with high-speed imaging and accelerometry, we have attempted to capture the effect of interfacial heterogeneity on cavitation thresholds in 7.5% w/v soft gelatin hydrogel. We have studied the role of the number of interfaces within a heterogenous gelatin column (homogeneous, two-layer, three-layer, five-layer) and their spatial location. Results indicate that the critical acceleration threshold for cavitation nucleation slightly decreases as the number of interfaces increases, with five-layer samples exhibiting the lowest threshold compared to the control homogeneous samples. Additional impact experiments are conducted on bi-material water-gel samples where the effect of interface orientation (vertical, horizontal, inclined) on cavitation is studied. The results show cavitation onset is delayed and duration is shorter in the gelatin region compared to the water region, regardless of orientation. These findings highlight the crucial role of interfacial characteristics in generating impact-induced cavitation in heterogeneous soft materials. Collectively, this study may provide valuable insights for designing materials with tailored cavitation resistance for biomedical and engineering applications.</p>