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<p><i>Asparagus racemosus</i> (Shatavari) is traditionally acclaimed for its galactagogue activity, yet the molecular basis and optimized isolation strategies for its major bioactive constituents remain insufficiently defined. This study reports an improved extraction and purification method yielding markedly higher quantities of Shatavarin IV (1.2% w/w) and Sarsasapogenin (0.5% w/w) compared with earlier reports (0.23% and 0.48% w/w). The optimized ethanol-based extraction and controlled acid hydrolysis facilitated efficient separation of saponins and sapogenins, while column chromatography was used to produce analytically pure compounds. The structural identity of the compounds was confirmed through FTIR, <sup>1</sup>H &amp; <sup>13</sup>C NMR, and MS, with diagnostic absorptions, characteristic proton and carbon resonances, and molecular ions (m/z 888.6 for Shatavarin IV; m/z 417 for Sarsasapogenin) matching reported data. HPTLC profiling demonstrated solvent-dependent distribution of phytoconstituents, supporting the extraction efficiency. Docking studies revealed higher PRLR binding affinities of Shatavarin IV and Sarsasapogenin than risperidone, supporting their potential role in JAK2/STAT5-mediated lactogenic activity of <i>A. racemosus</i> and highlight these molecules as promising PRLR-modulating candidates. The study provides a robust analytical and mechanistic foundation for standardizing <i>A. racemosus</i>–based formulations and advancing its steroidal saponins toward drug-development pipelines.</p>

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Isolation, spectral characterization, and in-silico lactogenic evaluation of Shatavarin IV and Sarsasapogenin from Asparagus racemosus Willd.

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月7日

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  • Ch Venkata Narasimhaji,
  • Murugammal Shanmugam,
  • D. Velvizhi,
  • Sujeet K. Mishra,
  • Yashika Gandhi,
  • Ravindra Singh,
  • Ajay Kumar Meena,
  • Narayanam Srikanth,
  • Rabinarayan Acharya

摘要

Asparagus racemosus (Shatavari) is traditionally acclaimed for its galactagogue activity, yet the molecular basis and optimized isolation strategies for its major bioactive constituents remain insufficiently defined. This study reports an improved extraction and purification method yielding markedly higher quantities of Shatavarin IV (1.2% w/w) and Sarsasapogenin (0.5% w/w) compared with earlier reports (0.23% and 0.48% w/w). The optimized ethanol-based extraction and controlled acid hydrolysis facilitated efficient separation of saponins and sapogenins, while column chromatography was used to produce analytically pure compounds. The structural identity of the compounds was confirmed through FTIR, 1H & 13C NMR, and MS, with diagnostic absorptions, characteristic proton and carbon resonances, and molecular ions (m/z 888.6 for Shatavarin IV; m/z 417 for Sarsasapogenin) matching reported data. HPTLC profiling demonstrated solvent-dependent distribution of phytoconstituents, supporting the extraction efficiency. Docking studies revealed higher PRLR binding affinities of Shatavarin IV and Sarsasapogenin than risperidone, supporting their potential role in JAK2/STAT5-mediated lactogenic activity of A. racemosus and highlight these molecules as promising PRLR-modulating candidates. The study provides a robust analytical and mechanistic foundation for standardizing A. racemosus–based formulations and advancing its steroidal saponins toward drug-development pipelines.