Scholarlink

<p>The nanopesticides are currently receiving great attention due to the several advantages reported for the use of nanoparticles. The study aimed to measure and evaluates the genotoxic effect of imidacloprid (I), nanoimidacloprid (IN), etofenprox (E) and nanoetofenprox (EN) on mice genome. Chitosan nanoparticles were used to prepare the nanoformulation of the two pesticides. Animals were divided into five groups and treated orally three times with interval period one week. The DNA fragmentation assay and Random Amplified Polymorphic DNA (RAPD) polymerase chain reaction analysis were used to evaluate the genotoxic effect of normal formulation and nano-formulation of imidacloprid and etofenprox on mice genome. The RAPD markers analysis showed that the imidacloprid and etofenprox in normal formulation and nano-formulation induced DNA alterations suggestive of genotoxicity in DNA genome screening compared to the control group which indicated to DNA damage at different molecular levels in treated groups. Estimation of the DNA damage by DNA fragmentation assay illustrated that the treated groups showed significant difference compared to the control. The nano-formulations of the two pesticides showed less DNA fragmentation compared to the normal formulation. The results revealed that the imidacloprid, nano-imidacloprid, etofenprox, and nano-etofenprox induced DNA alterations suggestive of genotoxicity.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genotoxicity characterization of imidacloprid and etofenprox nanoformulations in comparison with their normal formulations on mice

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月2日

查看全文
  • Al-Kazafy Hassan Sabry,
  • Reda Ismail Elsayad,
  • Sahar Ahmed

摘要

The nanopesticides are currently receiving great attention due to the several advantages reported for the use of nanoparticles. The study aimed to measure and evaluates the genotoxic effect of imidacloprid (I), nanoimidacloprid (IN), etofenprox (E) and nanoetofenprox (EN) on mice genome. Chitosan nanoparticles were used to prepare the nanoformulation of the two pesticides. Animals were divided into five groups and treated orally three times with interval period one week. The DNA fragmentation assay and Random Amplified Polymorphic DNA (RAPD) polymerase chain reaction analysis were used to evaluate the genotoxic effect of normal formulation and nano-formulation of imidacloprid and etofenprox on mice genome. The RAPD markers analysis showed that the imidacloprid and etofenprox in normal formulation and nano-formulation induced DNA alterations suggestive of genotoxicity in DNA genome screening compared to the control group which indicated to DNA damage at different molecular levels in treated groups. Estimation of the DNA damage by DNA fragmentation assay illustrated that the treated groups showed significant difference compared to the control. The nano-formulations of the two pesticides showed less DNA fragmentation compared to the normal formulation. The results revealed that the imidacloprid, nano-imidacloprid, etofenprox, and nano-etofenprox induced DNA alterations suggestive of genotoxicity.