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<p>Bio-priming with beneficial microbes has drawn the attention of researchers to address environmental constraints resulting from modern agricultural practices. To find the effect of bio-priming with <i>Trichoderma asperellum</i> on quality and quantity of sunflower oil, nucleic acid contents in leaf samples and to evaluate SSR markers for high oleic acid content in sunflower oil, an experiment was carried out in the department of Soil Science and Agricultural Chemistry, Institute of Agricultural Sciences, Banaras Hindu University during February to May, 2018 and 2019. All analyses were performed using standard protocols. Seed bio-priming with <i>Trichoderma asprellum</i> in combination with 80% of the recommended dose of fertilizer (RDF) led to a notable reduction in total saturated fatty acids in sunflower oil by 46% and 18% relative to the control and 100% RDF treatments, respectively. Conversely, this treatment increased the total unsaturated fatty acid content by 7.5% compared to the control and by 3% compared to the 100% RDF. Among all the treatments, seed bio-priming resulted in the lowest levels of palmitic acid (4.32%) and stearic acid (3.54%), while achieving the highest concentrations of unsaturated fatty acids, specifically linoleic acid (59.82%) and oleic acid (34.22%). To identify the high oleic acid treatments, 5 SSR markers were screened in which two markers generated bands and showed polymorphism. The maximum oleic acid content (34.22%) was observed in the treatment receiving 70% RDF combined with seed bio-priming using <i>Trichoderma asperellum</i>. This value was comparable to other bio-primed treatments and represented an increase of 9% and 15% over the control and 100% RDF treatments, respectively. This study integrated the seed bio-priming with <i>Trichoderma asperellum</i> and marker-assisted selection (MAS) using SSR markers to improve both the nutritional quality and genetic understanding of oleic acid enhancement in sunflower oil. Sunflower oil rich in monounsaturated fatty acids is more desirable and stable than standard sunflower oil. Bio-priming was proven to be a good tool to improve the oil quantity and oil quality of sunflower.</p>

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Oil quality and marker assisted selection for oleic acid content in response to seed bio-priming with Trichoderma asperellum in Helianthus annuus L.

  • 期刊论文

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  • 发表日期：

    2026年1月24日

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  • Siva Devika Ogireddy,
  • Sabyasachi Koley,
  • Jarupula Suman,
  • Amitava Rakshit

摘要

Bio-priming with beneficial microbes has drawn the attention of researchers to address environmental constraints resulting from modern agricultural practices. To find the effect of bio-priming with Trichoderma asperellum on quality and quantity of sunflower oil, nucleic acid contents in leaf samples and to evaluate SSR markers for high oleic acid content in sunflower oil, an experiment was carried out in the department of Soil Science and Agricultural Chemistry, Institute of Agricultural Sciences, Banaras Hindu University during February to May, 2018 and 2019. All analyses were performed using standard protocols. Seed bio-priming with Trichoderma asprellum in combination with 80% of the recommended dose of fertilizer (RDF) led to a notable reduction in total saturated fatty acids in sunflower oil by 46% and 18% relative to the control and 100% RDF treatments, respectively. Conversely, this treatment increased the total unsaturated fatty acid content by 7.5% compared to the control and by 3% compared to the 100% RDF. Among all the treatments, seed bio-priming resulted in the lowest levels of palmitic acid (4.32%) and stearic acid (3.54%), while achieving the highest concentrations of unsaturated fatty acids, specifically linoleic acid (59.82%) and oleic acid (34.22%). To identify the high oleic acid treatments, 5 SSR markers were screened in which two markers generated bands and showed polymorphism. The maximum oleic acid content (34.22%) was observed in the treatment receiving 70% RDF combined with seed bio-priming using Trichoderma asperellum. This value was comparable to other bio-primed treatments and represented an increase of 9% and 15% over the control and 100% RDF treatments, respectively. This study integrated the seed bio-priming with Trichoderma asperellum and marker-assisted selection (MAS) using SSR markers to improve both the nutritional quality and genetic understanding of oleic acid enhancement in sunflower oil. Sunflower oil rich in monounsaturated fatty acids is more desirable and stable than standard sunflower oil. Bio-priming was proven to be a good tool to improve the oil quantity and oil quality of sunflower.