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<p>Sheath blight (ShB) disease caused by the necrotrophic fungus <i>Rhizoctonia solani</i> significantly threatens global rice production. To date, most rice varieties are highly susceptible to ShB, which is largely influenced by susceptibility genes (S), especially those encoding SWEET proteins. In this study, we investigated the interaction between transcription factors associated with ShB susceptibility and the promoters of <i>OsSWEET11</i>, <i>OsSWEET13</i> and <i>OsSWEET14</i> genes in rice cultivars. A bioassay of eight rice cultivars demonstrated varying levels of resistance, with ASD16 exhibiting the highest susceptibility and IR50 displaying moderate resistance. In silico docking identified the transcription factor Os01g0954500 from the SRS family, which had the strongest binding affinity to <i>OsSWEET11</i> promoter with a Z-score of −&#xa0;2.2 and a HADDOCK score of −&#xa0;117.6 ± 11.1 in ASD16. In addition, 2D interaction studies revealed regulatory regions spanning within the promoter of <i>OsSWEET11</i> and play a pivotal role in ShB susceptibility. Overall, the present study provides a foundation for improving rice resistance to <i>R. solani</i> by targeting transcription factor-promoter interactions with advanced molecular interventions.</p>

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Integrated computational and in vivo approaches for promoter editing in SWEET genes to enhance sheath blight resistance in rice

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月30日

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  • Vignesh Ponnurangan,
  • David Jesudoss,
  • Varanavasiappan Shanmugam,
  • R. K. Mohana Pradeep,
  • Krish K. Kumar,
  • Jayakanthan Mannu,
  • Prakasam Vellaichamy,
  • Paranidharan Vaikuntavasan

摘要

Sheath blight (ShB) disease caused by the necrotrophic fungus Rhizoctonia solani significantly threatens global rice production. To date, most rice varieties are highly susceptible to ShB, which is largely influenced by susceptibility genes (S), especially those encoding SWEET proteins. In this study, we investigated the interaction between transcription factors associated with ShB susceptibility and the promoters of OsSWEET11, OsSWEET13 and OsSWEET14 genes in rice cultivars. A bioassay of eight rice cultivars demonstrated varying levels of resistance, with ASD16 exhibiting the highest susceptibility and IR50 displaying moderate resistance. In silico docking identified the transcription factor Os01g0954500 from the SRS family, which had the strongest binding affinity to OsSWEET11 promoter with a Z-score of − 2.2 and a HADDOCK score of − 117.6 ± 11.1 in ASD16. In addition, 2D interaction studies revealed regulatory regions spanning within the promoter of OsSWEET11 and play a pivotal role in ShB susceptibility. Overall, the present study provides a foundation for improving rice resistance to R. solani by targeting transcription factor-promoter interactions with advanced molecular interventions.