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<p>Colistin, also known as polymyxin E, is extensively used in veterinary and human medicine as a last line of defense against Gram-negative bacteria. Therefore, it is essential to monitor the development of resistance to this antibiotic to combat infections caused by multidrug-resistant bacteria. This study focuses on investigating the presence of the colistin resistance gene <i>mcr-1</i> in <i>Salmonella</i> isolates. A total of 24 <i>Salmonella</i> spp. were identified and analyzed from 200 cattle fecal samples. Phenotypic disc diffusion and a MIC assay using various antimicrobial agents were performed and assessed using the VITEK2 compact system. The isolates were further examined through PCR targeting the <i>invA</i> and <i>mcr-1</i> genes. Among the 24 isolates, two exhibited phenotypic resistance to colistin, while one isolate harbored the <i>mcr-1</i> gene. Notably, 14 (58.33%) of the 24 <i>Salmonella</i> isolates showed multidrug resistance. Furthermore, in-silico functional studies were conducted to explore the genetic relationships and phenotypic correlations among these colistin-resistant isolates. Sequencing and phylogenetic analysis revealed a significant evolutionary association between the catalytic region of <i>Neisseria meningitidis</i> EptA and MCR proteins. Structural modeling indicated that MCR-1 has five essential transmembrane regions for its function in colistin resistance. Molecular docking interaction studies demonstrated distinct binding mechanisms between MCR-1 and EptA proteins. This study highlights the occurrence of <i>mcr-1</i> carrying <i>Salmonella</i> in cattle from Bangladesh, underscoring the urgent need for robust AMR surveillance and stringent antibiotic use policies in agriculture to mitigate the spread of multidrug-resistant pathogens.</p>

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Structural function of multidrug-resistant Salmonella carrying mcr-1 gene in colistin resistance

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月10日

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  • Md. Mukter Hossain,
  • Riazul Islam Riad,
  • Md. Mashfiq Hossain Khadem,
  • Ruhena Begum,
  • Md. Shoriful Alam Sumon,
  • Ho-Seong Cho,
  • Md. Mahfujur Rahman,
  • Md Bashir Uddin

摘要

Colistin, also known as polymyxin E, is extensively used in veterinary and human medicine as a last line of defense against Gram-negative bacteria. Therefore, it is essential to monitor the development of resistance to this antibiotic to combat infections caused by multidrug-resistant bacteria. This study focuses on investigating the presence of the colistin resistance gene mcr-1 in Salmonella isolates. A total of 24 Salmonella spp. were identified and analyzed from 200 cattle fecal samples. Phenotypic disc diffusion and a MIC assay using various antimicrobial agents were performed and assessed using the VITEK2 compact system. The isolates were further examined through PCR targeting the invA and mcr-1 genes. Among the 24 isolates, two exhibited phenotypic resistance to colistin, while one isolate harbored the mcr-1 gene. Notably, 14 (58.33%) of the 24 Salmonella isolates showed multidrug resistance. Furthermore, in-silico functional studies were conducted to explore the genetic relationships and phenotypic correlations among these colistin-resistant isolates. Sequencing and phylogenetic analysis revealed a significant evolutionary association between the catalytic region of Neisseria meningitidis EptA and MCR proteins. Structural modeling indicated that MCR-1 has five essential transmembrane regions for its function in colistin resistance. Molecular docking interaction studies demonstrated distinct binding mechanisms between MCR-1 and EptA proteins. This study highlights the occurrence of mcr-1 carrying Salmonella in cattle from Bangladesh, underscoring the urgent need for robust AMR surveillance and stringent antibiotic use policies in agriculture to mitigate the spread of multidrug-resistant pathogens.