Scholarlink

<p>To address challenges associated with low remediation efficiency and susceptibility to environmental influences in free <i>Bacillus</i>, a polyvinyl alcohol (PVA)-sodium alginate (SA)-biochar (BC) adsorption-embedded immobilized <i>Bacillus</i> system was established. This system demonstrated a significant improvement in the degradation rate of petroleum hydrocarbons, reaching 66.41% after 120 days, contributing to the rehabilitation of petroleum-contaminated soil. This represented a notable increase of 26.41% and 14.41% compared to the rates achieved by free <i>Bacillus</i> and the biochar-immobilized <i>Bacillus</i> systems, respectively. Moreover, soil treated with the PVA-SA-BC adsorption-embedded immobilized <i>Bacillus</i> system exhibited higher dehydrogenase and catalase activities than those treated with free <i>Bacillus</i> and the biochar-immobilized <i>Bacillus</i> systems. The microbial community structures of different treatment groups were analyzed using high-throughput sequencing technology and the Analysis of Composition of Microbiomes with Bias Correction (ANCOM-BC) method. The results showed that adding free <i>Bacillus</i>, immobilized <i>Bacillus</i>, and the PVA-SA-BC adsorption-embedded immobilized <i>Bacillus</i> could significantly change the microbial community structure. Among them, the PVA-SA-BC adsorption-embedded immobilized <i>Bacillus</i> exhibited the most significant change in microbial abundance, with notable increases in the abundances of <i>Chungangia</i> and <i>Bacillus</i>. The adsorption-embedding immobilization technology provides a more stable environment for growth for exogenous <i>Bacillus</i>, thereby promoting the biodegradation of petroleum hydrocarbons more effectively. Spearman correlation analysis indicated that dehydrogenase activity, the abundance of <i>Bacillus</i>, and catalase activity were the key promoting factors for the degradation of petroleum hydrocarbons. This comprehensive investigation provides valuable insights into the application of the PVA-SA-BC adsorption-embedded immobilized <i>Bacillus</i> system for enhancing remediation efficiency in petroleum-contaminated soil.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Immobilized Bacillus in polyvinyl alcohol-sodium alginate-biochar for petroleum-contaminated soil remediation

  • 期刊论文

  • 发表日期：

    2026年3月3日

查看全文
  • Fenglei Han,
  • Zhoutong Liu,
  • Lihan Ren,
  • Biao Wang,
  • Fan Yang,
  • Feng Yao,
  • Xiaodong Zhao,
  • Xiuxia Zhang,
  • Kang Xiong,
  • De Li,
  • Wenhao Zuo

摘要

To address challenges associated with low remediation efficiency and susceptibility to environmental influences in free Bacillus, a polyvinyl alcohol (PVA)-sodium alginate (SA)-biochar (BC) adsorption-embedded immobilized Bacillus system was established. This system demonstrated a significant improvement in the degradation rate of petroleum hydrocarbons, reaching 66.41% after 120 days, contributing to the rehabilitation of petroleum-contaminated soil. This represented a notable increase of 26.41% and 14.41% compared to the rates achieved by free Bacillus and the biochar-immobilized Bacillus systems, respectively. Moreover, soil treated with the PVA-SA-BC adsorption-embedded immobilized Bacillus system exhibited higher dehydrogenase and catalase activities than those treated with free Bacillus and the biochar-immobilized Bacillus systems. The microbial community structures of different treatment groups were analyzed using high-throughput sequencing technology and the Analysis of Composition of Microbiomes with Bias Correction (ANCOM-BC) method. The results showed that adding free Bacillus, immobilized Bacillus, and the PVA-SA-BC adsorption-embedded immobilized Bacillus could significantly change the microbial community structure. Among them, the PVA-SA-BC adsorption-embedded immobilized Bacillus exhibited the most significant change in microbial abundance, with notable increases in the abundances of Chungangia and Bacillus. The adsorption-embedding immobilization technology provides a more stable environment for growth for exogenous Bacillus, thereby promoting the biodegradation of petroleum hydrocarbons more effectively. Spearman correlation analysis indicated that dehydrogenase activity, the abundance of Bacillus, and catalase activity were the key promoting factors for the degradation of petroleum hydrocarbons. This comprehensive investigation provides valuable insights into the application of the PVA-SA-BC adsorption-embedded immobilized Bacillus system for enhancing remediation efficiency in petroleum-contaminated soil.