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<p>The increasing dependence on fossil fuels, coupled with pressing environmental issues, and energy security challenges, has intensified the search for renewable and sustainable alternatives. Lignocellulosic biomass has emerged as a promising feedstock for biofuel ethanol production to <i>fuel</i> the transport sector. This study investigated the bioconversion potential of <i>Saccharum spontaneum</i> biomass (SSB) to biofuel ethanol in a one-pot consolidated bioprocess (OPCB) using a novel pretreatment regime based on deep eutectic solvent (choline chloride-glycerol) and microwave (MW) irradiation. Following pretreatment, the biomass polysaccharides were in situ hydrolysed with DES-compatible cellulase and xylanase enzymes derived from <i>Bacillus subtilis</i> G<sub>2</sub>, and the released sugars were in situ fermented to bioethanol with dual yeast cultures. Response surface methodology (RSM) based optimization of variables i.e. biomass loading (3.05%, w/w), pretreatment temperature (158.9ºC), and enzyme dosage (78.54&#xa0;mg/100&#xa0;mg biomass) significantly enhanced the sugar yield from initial 78.6&#xa0;mg/g to 384.7&#xa0;mg/g biomass (a 3.89-fold increase), thereby, enhancing the cellulose conversion efficiency from 13.84 to 67.76%. Thus, the results underscore the high pretreatment efficacy of biomass with combined DES and MW irradiation system. Ethanol fermentation under OPCB resulted in a yield of 148.1&#xa0;mg/g of biomass (bioconversion efficiency 51.3%). The consolidated bioprocessing represents an innovative and novel approach for efficient biomass conversion to biofuel; however, further process refinement must be executed for enhancing the bioconversion efficiency to realize the success of lignocellulosic biofuel technology.</p>

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Microwave-assisted deep eutectic solvent pretreatment of Saccharum spontaneum biomass for biofuel-ethanol production using one pot consolidated processing

  • 期刊论文

  • 发表日期：

    2025年6月13日

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  • Surbhi Vaid,
  • Surbhi Sharma,
  • Muskaan Chib,
  • Arpana Thakur,
  • Bisma Habib,
  • Mohd. Younis,
  • Nisha Kapoor,
  • Ritu Mahajan,
  • Bijender Kumar Bajaj

摘要

The increasing dependence on fossil fuels, coupled with pressing environmental issues, and energy security challenges, has intensified the search for renewable and sustainable alternatives. Lignocellulosic biomass has emerged as a promising feedstock for biofuel ethanol production to fuel the transport sector. This study investigated the bioconversion potential of Saccharum spontaneum biomass (SSB) to biofuel ethanol in a one-pot consolidated bioprocess (OPCB) using a novel pretreatment regime based on deep eutectic solvent (choline chloride-glycerol) and microwave (MW) irradiation. Following pretreatment, the biomass polysaccharides were in situ hydrolysed with DES-compatible cellulase and xylanase enzymes derived from Bacillus subtilis G2, and the released sugars were in situ fermented to bioethanol with dual yeast cultures. Response surface methodology (RSM) based optimization of variables i.e. biomass loading (3.05%, w/w), pretreatment temperature (158.9ºC), and enzyme dosage (78.54 mg/100 mg biomass) significantly enhanced the sugar yield from initial 78.6 mg/g to 384.7 mg/g biomass (a 3.89-fold increase), thereby, enhancing the cellulose conversion efficiency from 13.84 to 67.76%. Thus, the results underscore the high pretreatment efficacy of biomass with combined DES and MW irradiation system. Ethanol fermentation under OPCB resulted in a yield of 148.1 mg/g of biomass (bioconversion efficiency 51.3%). The consolidated bioprocessing represents an innovative and novel approach for efficient biomass conversion to biofuel; however, further process refinement must be executed for enhancing the bioconversion efficiency to realize the success of lignocellulosic biofuel technology.