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<p>Potato production in Ukraine is constrained by viral pathogens, particularly Potato virus Y (PVY) and Potato virus M (PVM), which are transmitted through vegetative propagation and aphid vectors. The aim of this study was to develop a PCR-based protocol for the detection of PVY and PVM and to assess virus distribution in different plant organs of cultivated potatoes. Samples from five Ukrainian potato cultivars were tested using specific primers targeting the coat protein genes of PVY and PVM. Amplification products of the expected sizes (~ 480&#xa0;bp for PVY and ~ 290&#xa0;bp for PVM) were consistently obtained, with sequencing confirming &gt; 98% identity to GenBank reference sequences. Real-time PCR analysis demonstrated reliable detection, with Ct values ranging from 23.18 to 31.44 depending on cultivar and tissue. Semi-quantitative PCR revealed organ-specific virus accumulation: PVY was detected primarily in leaves and stems, whereas PVM was most abundant in tubers. Mixed infections were observed in several cultivars, producing more severe symptoms than single infections. These findings highlight the importance of molecular diagnostics for virus detection, the need for tissue-specific sampling strategies, and the integration of PCR protocols into Ukrainian seed tuber certification programs to reduce virus dissemination and improve seed potato quality.</p>

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Detection and distribution analysis of potato virus Y (PVY) and potato virus M (PVM) in potato plant organs

  • 期刊论文

  • 发表日期：

    2026年7月29日

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  • Anton Gerilovych,
  • Nadiia Shevchenko,
  • Tetiana Oliynik

摘要

Potato production in Ukraine is constrained by viral pathogens, particularly Potato virus Y (PVY) and Potato virus M (PVM), which are transmitted through vegetative propagation and aphid vectors. The aim of this study was to develop a PCR-based protocol for the detection of PVY and PVM and to assess virus distribution in different plant organs of cultivated potatoes. Samples from five Ukrainian potato cultivars were tested using specific primers targeting the coat protein genes of PVY and PVM. Amplification products of the expected sizes (~ 480 bp for PVY and ~ 290 bp for PVM) were consistently obtained, with sequencing confirming > 98% identity to GenBank reference sequences. Real-time PCR analysis demonstrated reliable detection, with Ct values ranging from 23.18 to 31.44 depending on cultivar and tissue. Semi-quantitative PCR revealed organ-specific virus accumulation: PVY was detected primarily in leaves and stems, whereas PVM was most abundant in tubers. Mixed infections were observed in several cultivars, producing more severe symptoms than single infections. These findings highlight the importance of molecular diagnostics for virus detection, the need for tissue-specific sampling strategies, and the integration of PCR protocols into Ukrainian seed tuber certification programs to reduce virus dissemination and improve seed potato quality.