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<p>Yellow Mosaic Disease (YMD), caused by begomoviruses including mungbean yellow mosaic India virus (MYMIV) and mungbean yellow mosaic virus (MYMV), severely constrains blackgram (<i>Vigna mungo</i> L. Hepper) productivity in India. In this study, 36 diverse blackgram genotypes were evaluated for YMD resistance under natural hotspot conditions using the Percent Disease Index (PDI) at 60 days after sowing (DAS). PDI values ranged from 3.70% (DKU 116) to 77.78% (LBG 623), and the genotypes were categorised into resistant, moderately resistant, moderately susceptible, susceptible, and highly susceptible classes. A set of 25 Simple Sequence Repeat (SSR) markers was screened, of which 14 were polymorphic, with a mean Polymorphic Information Content (PIC) of 0.45. Marker-trait association analysis revealed a significant association between YMD resistance and the CEDG056_90 allele (Fisher’s Exact p = 0.00275; ANOVA p = 0.000012), which remained significant after Bonferroni correction for multiple testing (threshold p &lt; 0.00125 for 40 alleles). This allele was consistently found in resistant genotypes (DKU 116 and DKU 87) but was absent in the highly susceptible variety (LBG 623). Both Neighbour-joining (NJ) clustering and Principal Coordinate Analysis (PCoA) demonstrated a moderate relationship between molecular groupings and field-based resistance categories. The marker CEDG056_90 may be useful for marker-assisted selection (MAS) after validation in larger, independent populations.</p>

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Molecular characterization and field screening of Yellow Mosaic Disease (YMD) resistance in blackgram (Vigna mungo L. Hepper) using SSR markers

  • 期刊论文

  • 发表日期：

    2026年7月6日

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  • Dorka Vijaya Kumari Beri,
  • M. Indira,
  • G. Bindu Madhavi,
  • Roja Veeraghattapu

摘要

Yellow Mosaic Disease (YMD), caused by begomoviruses including mungbean yellow mosaic India virus (MYMIV) and mungbean yellow mosaic virus (MYMV), severely constrains blackgram (Vigna mungo L. Hepper) productivity in India. In this study, 36 diverse blackgram genotypes were evaluated for YMD resistance under natural hotspot conditions using the Percent Disease Index (PDI) at 60 days after sowing (DAS). PDI values ranged from 3.70% (DKU 116) to 77.78% (LBG 623), and the genotypes were categorised into resistant, moderately resistant, moderately susceptible, susceptible, and highly susceptible classes. A set of 25 Simple Sequence Repeat (SSR) markers was screened, of which 14 were polymorphic, with a mean Polymorphic Information Content (PIC) of 0.45. Marker-trait association analysis revealed a significant association between YMD resistance and the CEDG056_90 allele (Fisher’s Exact p = 0.00275; ANOVA p = 0.000012), which remained significant after Bonferroni correction for multiple testing (threshold p < 0.00125 for 40 alleles). This allele was consistently found in resistant genotypes (DKU 116 and DKU 87) but was absent in the highly susceptible variety (LBG 623). Both Neighbour-joining (NJ) clustering and Principal Coordinate Analysis (PCoA) demonstrated a moderate relationship between molecular groupings and field-based resistance categories. The marker CEDG056_90 may be useful for marker-assisted selection (MAS) after validation in larger, independent populations.