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<p><i>Ralstonia pseudosolanacearum</i>, a major pathogen causing bacterial wilt in various crops, persists in soil and water by entering a viable but non-culturable (VBNC) state under environmental stress. Conventional culture-based detection methods and existing molecular assays either lack species or physiological-state specificity or both. In this study we aimed to identify and evaluate <i>R. pseudosolanacearum</i>-specific mRNA markers expressed exclusively during the VBNC state. Among three targets evaluated using RT-PCR, the gene Rsc1857 was found distinctly expressed during the VBNC phase, with no detectable expression in culturable cells. The identified target demonstrated species and VBNC specificity. The practical applicability of the transcript Rsc1857 under field conditions was demonstrated by spike-in experiments in irrigation water. These findings put forward a novel approach for improving bacterial wilt monitoring and detection of <i>R. pseudosolanacearum</i> VBNC cells.</p>

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Molecular evaluation of viable but non-culturable (VBNC)-specific genes in Ralstonia pseudosolanacearum using RT-PCR

  • 期刊论文

  • 发表日期：

    2026年6月30日

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  • Jyotsana Maura,
  • Vineeta Bhatt,
  • Neha Faridi,
  • Merwyn Samuel,
  • Ankur Agarwal,
  • Anfal Arshi,
  • Suchita Atreya,
  • Divyesh Pathak,
  • Tuhina Sanwal,
  • Veena Pande

摘要

Ralstonia pseudosolanacearum, a major pathogen causing bacterial wilt in various crops, persists in soil and water by entering a viable but non-culturable (VBNC) state under environmental stress. Conventional culture-based detection methods and existing molecular assays either lack species or physiological-state specificity or both. In this study we aimed to identify and evaluate R. pseudosolanacearum-specific mRNA markers expressed exclusively during the VBNC state. Among three targets evaluated using RT-PCR, the gene Rsc1857 was found distinctly expressed during the VBNC phase, with no detectable expression in culturable cells. The identified target demonstrated species and VBNC specificity. The practical applicability of the transcript Rsc1857 under field conditions was demonstrated by spike-in experiments in irrigation water. These findings put forward a novel approach for improving bacterial wilt monitoring and detection of R. pseudosolanacearum VBNC cells.