<p>Seed rot and seedling blight of maize (<i>Zea mays</i> L.) caused by soil- and seed-borne fungi affects germination and early seedling establishment. In this study, the pathogenicity of <i>Fusarium verticillioides</i>, <i>Drechslera maydis</i>, <i>Rhizoctonia solani</i>, <i>Macrophomina phaseolina</i> and their consortium was evaluated at four inoculum levels (1 × 10⁴, 5 × 10⁴, 1 × 10⁵ and 5 × 10<sup>5</sup> spores/ml) using a seed dip inoculation technique. Germination, seedling survival and seedling blight severity were recorded at 0, 3 and 7 days after seed inoculation (DASI). Among the pathogens, <i>F. verticillioides</i> was the most virulent, reducing germination to 37.2% and seedling survival to 25% with maximum seedling blight severity of 90% at the highest inoculum level (7DASI). <i>Rhizoctonia solani</i> and <i>M. phaseolina</i> also caused severe disease, reducing germination to 64.2% and 64.4% and seedling survival to 30% and 35%, with maximum blight severity of 84% and 82%, respectively, while the pathogen consortium resulted in 82% seedling blight severity. In contrast, <i>D. maydis</i> showed comparatively lower pathogenicity. Disease severity increased with increasing inoculum concentration and incubation period, indicating that the seed dip inoculation method provides a reliable approach for evaluating pathogenic variability and determining inoculum thresholds for maize seedling blight pathogens.</p>

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Standardising seed dip inoculation: a key tool for assessing maize seedling blight

  • Bhawandeep Kaur,
  • Harleen Kaur

摘要

Seed rot and seedling blight of maize (Zea mays L.) caused by soil- and seed-borne fungi affects germination and early seedling establishment. In this study, the pathogenicity of Fusarium verticillioides, Drechslera maydis, Rhizoctonia solani, Macrophomina phaseolina and their consortium was evaluated at four inoculum levels (1 × 10⁴, 5 × 10⁴, 1 × 10⁵ and 5 × 105 spores/ml) using a seed dip inoculation technique. Germination, seedling survival and seedling blight severity were recorded at 0, 3 and 7 days after seed inoculation (DASI). Among the pathogens, F. verticillioides was the most virulent, reducing germination to 37.2% and seedling survival to 25% with maximum seedling blight severity of 90% at the highest inoculum level (7DASI). Rhizoctonia solani and M. phaseolina also caused severe disease, reducing germination to 64.2% and 64.4% and seedling survival to 30% and 35%, with maximum blight severity of 84% and 82%, respectively, while the pathogen consortium resulted in 82% seedling blight severity. In contrast, D. maydis showed comparatively lower pathogenicity. Disease severity increased with increasing inoculum concentration and incubation period, indicating that the seed dip inoculation method provides a reliable approach for evaluating pathogenic variability and determining inoculum thresholds for maize seedling blight pathogens.