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<p>The transcriptional dynamics of seven defence-related genes (chalcone synthase, PR-10, protein kinase, lipoxygenase, acidic glucanase, β-glucosidase, and cysteine proteinase) were profiled in chickpea cultivars contrasting in wilt resistance (GPF 2, resistant; JG 62, susceptible) during interactions with <i>Fusarium oxysporum</i> f. sp. <i>ciceris</i> (Foc) and the biocontrol agent <i>Trichoderma harzianum</i> (Pusa 5SD). Quantitative RT-PCR analysis revealed that <i>T. harzianum</i> priming, alone or in combination with Foc, significantly upregulated defence transcripts in both genotypes. Notably, the resistant cultivar exhibited rapid, high-magnitude gene induction (particularly CHS and PR-10) within 24–48&#xa0;h post-inoculation (hpi). Conversely, the susceptible cultivar displayed a delayed basal defence response (PTI) in Foc-alone treatments. However, <i>T. harzianum</i> application corrected this temporal latency in the susceptible host, enhancing the amplitude and speed of defence gene expression to levels comparable with the resistant genotype. These findings suggest that <i>T. harzianum</i> orchestrates a synergistic activation of salicylic acid (SA) and jasmonic acid (JA) pathways, effectively bolstering host immunity against vascular wilt.</p>

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Transcriptional modulation of defence genes by Trichoderma harzianum triggers induced systemic resistance in chickpea against Fusarium oxysporum f. sp. ciceris

  • 期刊论文

  • 发表日期：

    2026年3月8日

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  • Balendu K. Upadhyay,
  • Aditya Pratap Singh,
  • Kumari Priyanka,
  • Sunil C. Dubey,
  • Mathew S. Baite

摘要

The transcriptional dynamics of seven defence-related genes (chalcone synthase, PR-10, protein kinase, lipoxygenase, acidic glucanase, β-glucosidase, and cysteine proteinase) were profiled in chickpea cultivars contrasting in wilt resistance (GPF 2, resistant; JG 62, susceptible) during interactions with Fusarium oxysporum f. sp. ciceris (Foc) and the biocontrol agent Trichoderma harzianum (Pusa 5SD). Quantitative RT-PCR analysis revealed that T. harzianum priming, alone or in combination with Foc, significantly upregulated defence transcripts in both genotypes. Notably, the resistant cultivar exhibited rapid, high-magnitude gene induction (particularly CHS and PR-10) within 24–48 h post-inoculation (hpi). Conversely, the susceptible cultivar displayed a delayed basal defence response (PTI) in Foc-alone treatments. However, T. harzianum application corrected this temporal latency in the susceptible host, enhancing the amplitude and speed of defence gene expression to levels comparable with the resistant genotype. These findings suggest that T. harzianum orchestrates a synergistic activation of salicylic acid (SA) and jasmonic acid (JA) pathways, effectively bolstering host immunity against vascular wilt.