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<p><i>Pythium aphanidermatum</i>-induced pre- and post-emergence damping-off is a major soilborne oomycete disease in cucumber (<i>Cucumis sativus</i> L.). Biological control employing antagonistic microorganisms is considered one of the most efficient and sustainable methods for environmentally friendly management of cucumber damping-off (CDO). In this study, antagonistic bacteria associated with olive pomace compost (OPC) were isolated and evaluated against CDO. Of the 10 bacterial isolates evaluated, three isolates designated OPB1, OPB3, and OPB9 exerted strong antagonistic effects against <i>P. aphanidermatum</i> in an in vitro assay. These antagonists triggered morphological abnormalities in <i>P. aphanidermatum</i> hyphae as confirmed through scanning electron microscopy. The 16S rRNA gene sequencing identified these bacterial isolates as <i>Staphylococcus warneri</i> (OPB1), <i>S. haemolyticus</i> (OPB3) and <i>Pantoea dispersa</i> (OPB9). Soil drench application of <i>P. dispersa</i> OPB9 cell suspension significantly suppressed CDO under controlled glasshouse conditions. This bacterial isolate shows great promise as a potential bio-inoculant for the management of CDO in organic farming.</p>

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Pantoea dispersa from olive pomace compost suppresses Pythium aphanidermatum damping-off in Cucumis sativus

  • 期刊论文

  • 发表日期：

    2025年7月24日

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  • Ikhlass Al-Shaqsi,
  • Yahya Al-Fahdi,
  • Issa Hashil Al-Mahmooli,
  • Ibrahim Al-Khusaibi,
  • Ahmed Al-Fahdi,
  • Abdullah Mohammed Al-Sadi,
  • Rethinasamy Velazhahan

摘要

Pythium aphanidermatum-induced pre- and post-emergence damping-off is a major soilborne oomycete disease in cucumber (Cucumis sativus L.). Biological control employing antagonistic microorganisms is considered one of the most efficient and sustainable methods for environmentally friendly management of cucumber damping-off (CDO). In this study, antagonistic bacteria associated with olive pomace compost (OPC) were isolated and evaluated against CDO. Of the 10 bacterial isolates evaluated, three isolates designated OPB1, OPB3, and OPB9 exerted strong antagonistic effects against P. aphanidermatum in an in vitro assay. These antagonists triggered morphological abnormalities in P. aphanidermatum hyphae as confirmed through scanning electron microscopy. The 16S rRNA gene sequencing identified these bacterial isolates as Staphylococcus warneri (OPB1), S. haemolyticus (OPB3) and Pantoea dispersa (OPB9). Soil drench application of P. dispersa OPB9 cell suspension significantly suppressed CDO under controlled glasshouse conditions. This bacterial isolate shows great promise as a potential bio-inoculant for the management of CDO in organic farming.