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<p><i>Pseudomonas putida</i> BP25 is a black pepper endophytic bacterium exhibiting promising antifungal activity against several economically important plant pathogens especially <i>Phytophthora capsici</i>,<i> Magnaporthe oryzae</i>, and <i>Colletotrichum gloeosporioides</i>. In the present study, we report the complete genome sequence of <i>P. putida</i> BP25, comprising a single circular chromosome of 5.79&#xa0;Mb (Scaffold N50: 5777593&#xa0;bp; Contig N50:114500&#xa0;bp) with 5389 predicted genes and a G + C content of 62.92%. Average Nucleotide Identity (ANI) analysis indicated a high degree of similarity between <i>P. putida</i> BP25 and other beneficial endophytic strains of <i>P. putida</i>. Functional annotation revealed that the majority of pathway classes were associated with the biosynthesis of secondary metabolites (24 pathways), followed by xenobiotic biodegradation and metabolism (21 pathways). Notably, nine biosynthetic gene regions encompassing 11,168 secondary metabolite-coding clusters were identified through MiBiG analysis, suggesting the capacity of <i>P. putida</i> BP25 to produce a range of antimicrobial compounds. Collectively, genome analysis, along with previous transcriptomic and elicitor-based studies, underscores the potential of <i>P. putida</i> BP25 as a biocontrol agent and its application in reducing chemical inputs in sustainable agriculture.</p> Graphical Abstract <p></p>

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Genomic insights into endophytic Pseudomonas putida BP25: deciphering antifungal capabilities and secondary metabolite synthesis for enhanced plant disease management

  • 期刊论文

  • 发表日期：

    2025年5月7日

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  • Mushineni Ashajyothi,
  • Shanmugam Velmurugan,
  • Neelam Sheoran,
  • Asharani Patel,
  • Shanu Kumar,
  • K. Charishma,
  • Aundy Kumar

摘要

Pseudomonas putida BP25 is a black pepper endophytic bacterium exhibiting promising antifungal activity against several economically important plant pathogens especially Phytophthora capsici, Magnaporthe oryzae, and Colletotrichum gloeosporioides. In the present study, we report the complete genome sequence of P. putida BP25, comprising a single circular chromosome of 5.79 Mb (Scaffold N50: 5777593 bp; Contig N50:114500 bp) with 5389 predicted genes and a G + C content of 62.92%. Average Nucleotide Identity (ANI) analysis indicated a high degree of similarity between P. putida BP25 and other beneficial endophytic strains of P. putida. Functional annotation revealed that the majority of pathway classes were associated with the biosynthesis of secondary metabolites (24 pathways), followed by xenobiotic biodegradation and metabolism (21 pathways). Notably, nine biosynthetic gene regions encompassing 11,168 secondary metabolite-coding clusters were identified through MiBiG analysis, suggesting the capacity of P. putida BP25 to produce a range of antimicrobial compounds. Collectively, genome analysis, along with previous transcriptomic and elicitor-based studies, underscores the potential of P. putida BP25 as a biocontrol agent and its application in reducing chemical inputs in sustainable agriculture.

Graphical Abstract