Scholarlink

<p>To develop a PCR-based diagnostic method for plant DNA virus diseases, the applicability of a DNA purification-free PCR method was evaluated for detecting a notorious bipartite begomovirus, pepper yellow leaf curl virus (PepYLCV). DNA extraction was carried out by commercial kit, one-tube DNA extraction method and DNA purification-free method or homemade DNA storage paper-mediated PCR (HDP-PCR) to detect the presence of PepYLCV in field samples. Comparative analysis showed that all three methods successfully detected PepYLCV in field samples by amplifying the target 912&#xa0;bp fragment through PCR. The one-tube DNA extraction method successfully detected the virus in all tested samples, including those extracted on the sampling day and those stored at room temperature for one week. The HDP-PCR method detected the presence of pepper yellow leaf curl virus in the fresh as well as two-month-old filter paper samples, indicating the stability of viral DNA in filter paper. Further, the HDP-PCR method is the most cost- and time-effective out of the three methods used for DNA extraction.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Detection of pepper yellow leafcurl virus in chilli plants by DNA purification-free PCR method

  • 期刊论文

  • 发表日期：

    2025年4月15日

查看全文
  • Hamdayanty,
  • Tutik Kuswinanti,
  • Mustika Tuwo,
  • Hidetaka Kaya,
  • Kappei Kobayashi

摘要

To develop a PCR-based diagnostic method for plant DNA virus diseases, the applicability of a DNA purification-free PCR method was evaluated for detecting a notorious bipartite begomovirus, pepper yellow leaf curl virus (PepYLCV). DNA extraction was carried out by commercial kit, one-tube DNA extraction method and DNA purification-free method or homemade DNA storage paper-mediated PCR (HDP-PCR) to detect the presence of PepYLCV in field samples. Comparative analysis showed that all three methods successfully detected PepYLCV in field samples by amplifying the target 912 bp fragment through PCR. The one-tube DNA extraction method successfully detected the virus in all tested samples, including those extracted on the sampling day and those stored at room temperature for one week. The HDP-PCR method detected the presence of pepper yellow leaf curl virus in the fresh as well as two-month-old filter paper samples, indicating the stability of viral DNA in filter paper. Further, the HDP-PCR method is the most cost- and time-effective out of the three methods used for DNA extraction.