Scholarlink

<p>Pathogenic species of the genus <i>Venturia</i>, which cause scab diseases, significantly impact pome and stone fruit production. Simple sequence repeats (SSRs) are widely used in molecular studies due to their neutrality and high polymorphism, aiding in population genetics and evolutionary analyses. However, only a few species-specific SSR markers have been developed for <i>Venturia</i>, highlighting the need for a genus-wide cross-transferable SSR marker set. This study mined 116,768 SSRs from 22 genomes of seven <i>Venturia</i> species. Di-, tri-, and hexa-nucleotide repeats accounted for over 85% of SSRs in each genome. A core set of 10 cross-transferable SSR markers was identified, with seven markers validated <i>in vitro</i> for their high polymorphism in four <i>Venturia</i> populations. Among these, primer C199 (CT22) had the highest polymorphism information content (PIC), while NAPR45 (CT23) exhibited the most polymorphic alleles. AMOVA revealed that 52.87% of the genetic variation existed within populations, while 47.13% was among populations. The fixation index (<i>F</i><sub><i>ST</i></sub> = 0.47) and clustering analysis (STRUCTURE, K = 2) confirmed significant differentiation. The index of association indicated clonal behavior in apple and almond populations, whereas pear and peach populations were more diverse. This first genus-wide cross-transferable SSR marker set for <i>Venturia</i> offers a robust tool for genetic studies, especially in scab-causing pathogens of pome and stone fruits.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

High-resolution microsatellite profiling and development of universal cross-transferable markers for the genus Venturia

  • 期刊论文

  • 发表日期：

    2025年3月14日

查看全文
  • Syed Shoaib Mubashir,
  • M. S. Dar,
  • N. A. Khan,
  • Aasiya Nabi,
  • Bilal A. Padder

摘要

Pathogenic species of the genus Venturia, which cause scab diseases, significantly impact pome and stone fruit production. Simple sequence repeats (SSRs) are widely used in molecular studies due to their neutrality and high polymorphism, aiding in population genetics and evolutionary analyses. However, only a few species-specific SSR markers have been developed for Venturia, highlighting the need for a genus-wide cross-transferable SSR marker set. This study mined 116,768 SSRs from 22 genomes of seven Venturia species. Di-, tri-, and hexa-nucleotide repeats accounted for over 85% of SSRs in each genome. A core set of 10 cross-transferable SSR markers was identified, with seven markers validated in vitro for their high polymorphism in four Venturia populations. Among these, primer C199 (CT22) had the highest polymorphism information content (PIC), while NAPR45 (CT23) exhibited the most polymorphic alleles. AMOVA revealed that 52.87% of the genetic variation existed within populations, while 47.13% was among populations. The fixation index (FST = 0.47) and clustering analysis (STRUCTURE, K = 2) confirmed significant differentiation. The index of association indicated clonal behavior in apple and almond populations, whereas pear and peach populations were more diverse. This first genus-wide cross-transferable SSR marker set for Venturia offers a robust tool for genetic studies, especially in scab-causing pathogens of pome and stone fruits.