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<p>The rapid advancement of nanotechnology has highlighted the potential of copper oxide nanoparticles (CuO NPs) due to their wide industrial and commercial applications. In the present study, CuO nanoparticles were synthesized through an eco-friendly green synthesis approach using the floral extract of <i>Calotropis gigantea</i> (family: Apocynaceae). This plant was known to possess diverse pharmacological properties attributed to its rich content of polar phytochemicals such as flavonoids, alkaloids, terpenoids, tannins, phenolics, and saponins. The green synthesis process involved the reaction between a copper ion donor salt and the aqueous flower extract of <i>C. gigantea</i>, which facilitated the bio-reduction and stabilization of CuO nanoparticles. The use of <i>C. gigantea</i> for nanoparticle synthesis offered several advantages over conventional methods, including safety, cost-effectiveness, environmental sustainability, and biocompatibility. The DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging experiment was used to evaluate the antioxidant capability of the produced CuO nanoparticles. which demonstrated significant, concentration-dependent free radical inhibition. The antibacterial efficacy of the produced CuO nanoparticle against particular Gram positive and Gram-negative microorganisms was also assessed. Several microscopic and spectroscopic methods, including X-ray diffraction (XRD), Fourier- transform infrared spectroscopy (FTIR), UV-Visible spectrophotometry, scanning electron microscopy (SEM) and Transmission electron microscopy (TEM), were used to confirm the successful formation and characterization of CuO NPs mediated by C. gigantea.</p>

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Floral- Mediated Synthesis of Copper Oxide Nanoparticles via Calotropis gigantea: Structural Insights, Antimicrobial and Antioxidant Activity

  • 期刊论文

  • 发表日期：

    2026年6月24日

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  • Manisha Sonkusare,
  • Sanjogta Meshram,
  • Sanyogita Verma

摘要

The rapid advancement of nanotechnology has highlighted the potential of copper oxide nanoparticles (CuO NPs) due to their wide industrial and commercial applications. In the present study, CuO nanoparticles were synthesized through an eco-friendly green synthesis approach using the floral extract of Calotropis gigantea (family: Apocynaceae). This plant was known to possess diverse pharmacological properties attributed to its rich content of polar phytochemicals such as flavonoids, alkaloids, terpenoids, tannins, phenolics, and saponins. The green synthesis process involved the reaction between a copper ion donor salt and the aqueous flower extract of C. gigantea, which facilitated the bio-reduction and stabilization of CuO nanoparticles. The use of C. gigantea for nanoparticle synthesis offered several advantages over conventional methods, including safety, cost-effectiveness, environmental sustainability, and biocompatibility. The DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging experiment was used to evaluate the antioxidant capability of the produced CuO nanoparticles. which demonstrated significant, concentration-dependent free radical inhibition. The antibacterial efficacy of the produced CuO nanoparticle against particular Gram positive and Gram-negative microorganisms was also assessed. Several microscopic and spectroscopic methods, including X-ray diffraction (XRD), Fourier- transform infrared spectroscopy (FTIR), UV-Visible spectrophotometry, scanning electron microscopy (SEM) and Transmission electron microscopy (TEM), were used to confirm the successful formation and characterization of CuO NPs mediated by C. gigantea.