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<p>Date (<i>Phoenix dactylifera</i> L.) seeds have gained attention as a valuable co-product for the date fruit industry and were recognized as a rich source of functional and bioactive compounds. In this study, a date seed decoction (w/v = 1/10, temperature = 100&#xa0;°C, time = 15&#xa0;min) and a green solvent extraction (w/v = 1/10, temperature = 40&#xa0;°C, time = 3&#xa0;h) were applied to Deglet Enour date seeds using water–ethanol, water–acetone, or a water–acetone-ethanol mixture. The phytochemical content of the different date seed extracts was quantified spectrophotometrically and characterized using ATR-FTIR. The antioxidant, antibacterial and antibiofilm activities of the extracts against foodborne pathogenic bacteria were evaluated. Additionally, the potential prebiotic effect of date seed extract on <i>Lactobacillus gasseri</i> ADG1b was investigated. The water–acetone-ethanol extract exhibited the highest levels of total phenols, flavonoids, tannins, and carotenoids. All tested extracts demonstrated strong antioxidant activity, ranging from 2.69 ± 0.00 to 3.07 ± 0.01&#xa0;g TE/100&#xa0;g DM in the DPPH assay,12.64 ± 0.30 to 12.81 ± 0.45&#xa0;g TE/100&#xa0;g DM in the ABTS assay, and 1.28 ± 1.05 to2.01 ± 0.33&#xa0;g TE/100&#xa0;g DM in the FRAP assay. The water–acetone-ethanol extract (0.1–50&#xa0;mg/mL) exhibited significant antibacterial effects and effectively inhibited biofilm formation in tested foodborne pathogenic strains in a dose-dependent manner. Moreover, this extract demonstrated promising prebiotic potential at 0.1% on <i>L. gasseri</i> ADG1b. UHPLC-DAD-ESI/MS analysis identified epicatechin and procyanidins as the major phenolic compounds in the ternary solvent mixture.</p>

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Polyphenols-rich date seeds extracts: A multifunctional antioxidant with antibacterial, antibiofilm and prebiotic properties enhancing Lactobacillus gasseri growth

  • 期刊论文

  • 发表日期：

    2026年2月16日

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  • Amal Dbeibia,
  • Ramla Khiari,
  • Nahla Zeghonda,
  • Rafik Balti,
  • Nourhene Boudhrioua

摘要

Date (Phoenix dactylifera L.) seeds have gained attention as a valuable co-product for the date fruit industry and were recognized as a rich source of functional and bioactive compounds. In this study, a date seed decoction (w/v = 1/10, temperature = 100 °C, time = 15 min) and a green solvent extraction (w/v = 1/10, temperature = 40 °C, time = 3 h) were applied to Deglet Enour date seeds using water–ethanol, water–acetone, or a water–acetone-ethanol mixture. The phytochemical content of the different date seed extracts was quantified spectrophotometrically and characterized using ATR-FTIR. The antioxidant, antibacterial and antibiofilm activities of the extracts against foodborne pathogenic bacteria were evaluated. Additionally, the potential prebiotic effect of date seed extract on Lactobacillus gasseri ADG1b was investigated. The water–acetone-ethanol extract exhibited the highest levels of total phenols, flavonoids, tannins, and carotenoids. All tested extracts demonstrated strong antioxidant activity, ranging from 2.69 ± 0.00 to 3.07 ± 0.01 g TE/100 g DM in the DPPH assay,12.64 ± 0.30 to 12.81 ± 0.45 g TE/100 g DM in the ABTS assay, and 1.28 ± 1.05 to2.01 ± 0.33 g TE/100 g DM in the FRAP assay. The water–acetone-ethanol extract (0.1–50 mg/mL) exhibited significant antibacterial effects and effectively inhibited biofilm formation in tested foodborne pathogenic strains in a dose-dependent manner. Moreover, this extract demonstrated promising prebiotic potential at 0.1% on L. gasseri ADG1b. UHPLC-DAD-ESI/MS analysis identified epicatechin and procyanidins as the major phenolic compounds in the ternary solvent mixture.