Scholarlink

<p>Fractures constitute a major global health burden, affecting individuals across all demographic groups. A significant clinical challenge is delayed fracture healing, which often requires pharmacological intervention to enhance bone regeneration. Alkaline phosphatase (ALP), a key enzymatic biomarker of osteoblastic activity, is widely used to monitor bone repair progression and evaluate therapeutic efficacy. However, current ALP quantification methods are limited by high costs and low detection sensitivity, significantly restricting their clinical utility in fracture management. To address these limitations, we developed an ultrasensitive immunosensor based on a g-C<sub>3</sub>N<sub>4</sub>/Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> MXene/DWCNT-COOH nanocomposite. Although g-C<sub>3</sub>N<sub>4</sub> exhibits poor intrinsic conductivity, we incorporated Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> MXene as a functional additive to assemble with g-C<sub>3</sub>N<sub>4</sub>, creating a conductive platform that enhances electron transfer at the electrode interface and amplifies the electrochemical signal. Furthermore, the introduction of DWCNT-COOH not only synergistically improves electrochemical performance but also forms carboxy-amide bonds with amino groups in subsequent antibodies, offering numerous binding sites for ALP antibodies. This immunosensor utilizes differential pulse voltammetry (DPV) for electrochemical analysis, achieving a detection range of 10<sup>− 13</sup> to 10<sup>− 9</sup> g/mL and a remarkably low detection limit of 1.05 × 10<sup>− 14</sup>&#xa0;g/mL. Compared to existing methods, this immunosensor demonstrates superior sensitivity, making it a promising tool for clinical applications.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

An Sensitive Electrochemical Immunosensor for the Detection of Alkaline Phosphatase Based on g-C3N4/Ti3C2Tx MXene/DWCNT-COOH Nanocomposite

  • 期刊论文

  • 发表日期：

    2025年8月11日

查看全文
  • Zemei Mao,
  • Zhiruo Yang,
  • Lu Wang,
  • Nicole Jaffrezic-Renault,
  • Zhenzhong Z. Guo

摘要

Fractures constitute a major global health burden, affecting individuals across all demographic groups. A significant clinical challenge is delayed fracture healing, which often requires pharmacological intervention to enhance bone regeneration. Alkaline phosphatase (ALP), a key enzymatic biomarker of osteoblastic activity, is widely used to monitor bone repair progression and evaluate therapeutic efficacy. However, current ALP quantification methods are limited by high costs and low detection sensitivity, significantly restricting their clinical utility in fracture management. To address these limitations, we developed an ultrasensitive immunosensor based on a g-C3N4/Ti3C2Tx MXene/DWCNT-COOH nanocomposite. Although g-C3N4 exhibits poor intrinsic conductivity, we incorporated Ti3C2Tx MXene as a functional additive to assemble with g-C3N4, creating a conductive platform that enhances electron transfer at the electrode interface and amplifies the electrochemical signal. Furthermore, the introduction of DWCNT-COOH not only synergistically improves electrochemical performance but also forms carboxy-amide bonds with amino groups in subsequent antibodies, offering numerous binding sites for ALP antibodies. This immunosensor utilizes differential pulse voltammetry (DPV) for electrochemical analysis, achieving a detection range of 10− 13 to 10− 9 g/mL and a remarkably low detection limit of 1.05 × 10− 14 g/mL. Compared to existing methods, this immunosensor demonstrates superior sensitivity, making it a promising tool for clinical applications.