<p>The medicinal plants of the <i>Artemisia</i> genus [Asteraceae] are broadly distributed in large areas of Iran and are well-known for the vast diversity of their secondary metabolites and pharmacological effects. This study was aimed at phytochemically investigating the aerial parts of <i>Artemisia marschalliana</i> in an effort to isolate pure compounds and evaluate the plant’s anti-oxidant, anti-malarial, and anti-microbial effects. The aerial parts of <i>A. marschalliana</i> were solvent extracted with <i>n-</i>hexane, dichloromethane, and methanol (MeOH), respectively, via a soxhlet device. The MeOH extract was further fractionated via solid-phase extraction. The purification of phytochemicals was accomplished through reversed-phase high-performance liquid chromatography. For elucidation of the isolated chemical structures, ¹H-NMR and ¹³C-NMR spectra were scrutinized. Investigations conducted on <i>A. marschalliana</i> to isolate its chemical compounds led to the isolation of a phenylethanoid compound named salidroside, a phenolic compound known as tachioside, and a glucoside compound named 4-hydroxybenzoic acid 4-<i>O</i>-glucoside. The highest anti-oxidant activity was assigned to the MeOH extract and its 20% and 40% Sep-pak fractions, respectively. The crude MeOH extract exhibited the ultimate anti-malarial activity, to the extent that it was comparable to the highly endorsed anti-malarial drug chloroquine. The 40% and 10% MeOH Sep-pak fractions held the topmost anti-malarial effects. Moreover, among the crude extracts, the <i>n-</i>hexane extract demonstrated the maximum anti-microbial activity.</p> Graphical Abstract <p></p>