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<p>The goal of this research was to evaluate the effect of pressure variation and co-solvent addition in the supercritical carbon dioxide (SC-CO<sub>2</sub>) extraction of bioactive compounds from <i>Eucalyptus</i> leaves regarding yields, total phenolic contents (TPC), chemical profile, and biological activities of different extracts. The pressure was varied from 90 to 800&#xa0;bar at a fixed temperature of 40 ±  ± °C. The effect of co-solvent was evaluated at 5% (v/v) of ethanol. The best pressure for increasing yield (4.45%) and TPC (36.2&#xa0;mg gallic acid equivalent/g dry weight) was 400&#xa0;bar. The co-solvent addition increased the yield by 2.2% and the TPC by 54.5&#xa0;mg gallic acid equivalent/g dry weight (mg GAE/g DW). Gas chromatography-mass spectrometry (GC–MS) analysis revealed that delta cadinene and caryophyllene oxide were the main compounds at 90&#xa0;bar. Longifolene and cerotic acid were the major compounds detected in all other extracts. High-performance liquid chromatography (HPLC) showed that 3′-hydroxy-6-methylflavone (78.47&#xa0;mg/g), 5-hydroxy-3'-methoxyflavone (12.61&#xa0;mg/g), and cardamonin. (11.72&#xa0;mg/g) were the main compounds detected in the different extracts. The most important inhibition percentage (IP) of 61% against 5-lipoxygenase was obtained at 400&#xa0;bar<b>.</b> Whereas, the highest antioxidant activity (IP = 26%), as well as the strongest xanthine oxidase inhibition (56%),&#xa0;was obtained at 120&#xa0;bar. All extracts revealed a potential cytotoxic capacity against human breast, colon, and ovarian cancer cell lines with a promising inhibition concentration (IC50), achieving 3&#xa0;mg/L. Overall, processing at high pressure or with a co-solvent was successfully used to extract new active molecules from <i>E. diversifolia</i> intended for applications in the pharmaceutical industry.</p>

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Supercritical CO2 Extraction of Tunisian Eucalyptus diversifolia: Effects of Pressure and Co-solvent Addition

  • 期刊论文

  • 发表日期：

    2025年2月10日

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  • Saousan Chamali,
  • Jalloul Bouajila,
  • Severine Camy,
  • Ezzeddine Saadaoui,
  • Jean-Stephane Condoret,
  • Mehrez Romdhane

摘要

The goal of this research was to evaluate the effect of pressure variation and co-solvent addition in the supercritical carbon dioxide (SC-CO2) extraction of bioactive compounds from Eucalyptus leaves regarding yields, total phenolic contents (TPC), chemical profile, and biological activities of different extracts. The pressure was varied from 90 to 800 bar at a fixed temperature of 40 ±  ± °C. The effect of co-solvent was evaluated at 5% (v/v) of ethanol. The best pressure for increasing yield (4.45%) and TPC (36.2 mg gallic acid equivalent/g dry weight) was 400 bar. The co-solvent addition increased the yield by 2.2% and the TPC by 54.5 mg gallic acid equivalent/g dry weight (mg GAE/g DW). Gas chromatography-mass spectrometry (GC–MS) analysis revealed that delta cadinene and caryophyllene oxide were the main compounds at 90 bar. Longifolene and cerotic acid were the major compounds detected in all other extracts. High-performance liquid chromatography (HPLC) showed that 3′-hydroxy-6-methylflavone (78.47 mg/g), 5-hydroxy-3'-methoxyflavone (12.61 mg/g), and cardamonin. (11.72 mg/g) were the main compounds detected in the different extracts. The most important inhibition percentage (IP) of 61% against 5-lipoxygenase was obtained at 400 bar. Whereas, the highest antioxidant activity (IP = 26%), as well as the strongest xanthine oxidase inhibition (56%), was obtained at 120 bar. All extracts revealed a potential cytotoxic capacity against human breast, colon, and ovarian cancer cell lines with a promising inhibition concentration (IC50), achieving 3 mg/L. Overall, processing at high pressure or with a co-solvent was successfully used to extract new active molecules from E. diversifolia intended for applications in the pharmaceutical industry.