Scholarlink

<p>Diabetic wounds pose a major clinical problem through compromised healing processes with oxidative stress, chronic inflammation, and deranged tissue remodeling. In this research, a new stimuli-responsive hydrogel was prepared by adding <i>Bergenia ciliata</i> extract and its silver nanoparticles to <i>Salvia hispanica</i> (chia seed) mucilage. The hydrogel containing <i>Bergenia ciliata</i> extract and hydrogel containing BCNPs were characterized by UV-Vis spectroscopy and Fourier-transform infrared (FTIR) analysis. The presence of peaks and peak shifts at 280&#xa0;nm, 370&#xa0;nm, and 420&#xa0;nm in UV-vis and FTIR (500–4000&#xa0;cm<sup>− 1</sup>) confirmed the incorporation of extract and the presence of various functional groups at different peaks. The hydrogel demonstrated ion and pH-responsive swelling behaviour, with minimum swelling in ethanol, NaCl, AlCl₃, and MgSO₄ and maximum swelling in distilled water and KCl. Its appropriateness for dynamic wound microenvironments is highlighted by its regulated swelling–deswelling adaptation to changing pH and ionic circumstances. In vivo contraction of wounds at days 3, 6, and 9 demonstrated significantly expedited healing in hydrogel integrated BCNPs-treated diabetic models versus diabetic negative control (DNC)group. These results were further strengthened by the restoration of these biomarkers like GSH (16.1 ± 1.2 umol/L), CAT (3.9 ± 0.1 mmol/L), SOD (178.0 ± 3.4 U/ml), GPx (207.6 ± 4.3 U/L), TIMPs (173.0 ± 8.0 pg/ml), and IL-10 (50.5 ± 1.8 pg/ml) and significantly reduce the levels of MMP2 (216.0 ± 11.6 pg/ml), MMP7 (251.4 ± 9.9 pg/ml), MMP9 (159.8 ± 12.2 pg/ml), TNF-α (15.2 ± 0.4 pg/ml), IL-6 (15.0 ± 0.7 pg/ml), IL-8 (15.6 ± 1.2 pg/ml), IL-1β (153.0 ± 3.1 pg/ml) as compared to DNC group. The histopathological analysis ascertained increased re-epithelialization, neovascularization, and collagen organization. Cumulatively, these rich therapeutic properties make the <i>Bergenia ciliata</i> incorporated <i>Salvia hispanica</i> hydrogel a potential bioactive wound dressing with enhanced efficacy for diabetic wound healing, an area with potential clinical application.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Stimuli-responsive Bergenia ciliata-loaded hydrogel accelerates diabetic wound healing via antioxidant and anti-inflammatory mechanisms by subsequent bioactive release

  • 期刊论文

  • 发表日期：

    2026年3月26日

查看全文
  • Memoona Ashfaq,
  • Shaukat Ali,
  • Muhammad Summer

摘要

Diabetic wounds pose a major clinical problem through compromised healing processes with oxidative stress, chronic inflammation, and deranged tissue remodeling. In this research, a new stimuli-responsive hydrogel was prepared by adding Bergenia ciliata extract and its silver nanoparticles to Salvia hispanica (chia seed) mucilage. The hydrogel containing Bergenia ciliata extract and hydrogel containing BCNPs were characterized by UV-Vis spectroscopy and Fourier-transform infrared (FTIR) analysis. The presence of peaks and peak shifts at 280 nm, 370 nm, and 420 nm in UV-vis and FTIR (500–4000 cm− 1) confirmed the incorporation of extract and the presence of various functional groups at different peaks. The hydrogel demonstrated ion and pH-responsive swelling behaviour, with minimum swelling in ethanol, NaCl, AlCl₃, and MgSO₄ and maximum swelling in distilled water and KCl. Its appropriateness for dynamic wound microenvironments is highlighted by its regulated swelling–deswelling adaptation to changing pH and ionic circumstances. In vivo contraction of wounds at days 3, 6, and 9 demonstrated significantly expedited healing in hydrogel integrated BCNPs-treated diabetic models versus diabetic negative control (DNC)group. These results were further strengthened by the restoration of these biomarkers like GSH (16.1 ± 1.2 umol/L), CAT (3.9 ± 0.1 mmol/L), SOD (178.0 ± 3.4 U/ml), GPx (207.6 ± 4.3 U/L), TIMPs (173.0 ± 8.0 pg/ml), and IL-10 (50.5 ± 1.8 pg/ml) and significantly reduce the levels of MMP2 (216.0 ± 11.6 pg/ml), MMP7 (251.4 ± 9.9 pg/ml), MMP9 (159.8 ± 12.2 pg/ml), TNF-α (15.2 ± 0.4 pg/ml), IL-6 (15.0 ± 0.7 pg/ml), IL-8 (15.6 ± 1.2 pg/ml), IL-1β (153.0 ± 3.1 pg/ml) as compared to DNC group. The histopathological analysis ascertained increased re-epithelialization, neovascularization, and collagen organization. Cumulatively, these rich therapeutic properties make the Bergenia ciliata incorporated Salvia hispanica hydrogel a potential bioactive wound dressing with enhanced efficacy for diabetic wound healing, an area with potential clinical application.

Graphical Abstract