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<p>​The present study aimed to develop and assess a chitosan oligosaccharide (COS)-based ternary composite for enhanced wound healing using an integrated experimental and computational approach. A glutaraldehyde crosslinked COS, nano-bentonite (N-Bent), and polyethylene glycol (PEG) ternary composite in a 2:1:1 ratio was prepared and characterised using FTIR, XRD, SEM, particle size analysis, and swelling studies. The composite exhibited strong antioxidant activity in DPPH, NO, H<sub>2</sub>O<sub>2</sub>, and ABTS assays, with an antioxidant activity index of 7.47. It also demonstrated significant antibacterial and antifungal properties compared to standard drugs. Cytocompatibility of the composite with L929 fibroblast cells was confirmed using the MTT assay. The scratch wound healing test revealed the composite's ability to promote fibroblast migration with more than 92% wound closure in 36&#xa0;h and significant collagen formation, indicating its potential in wound healing applications. Computational studies using molecular docking showed the composite's interaction with key wound hfaling targets such as TNF-alpha, TGF beta RI, interleukin 1 beta, and <i>Pseudomonas aeruginosa</i>, suggesting its dual action of antibacterial and wound-healing activity. The integrated experimental and computational findings highlight the promising potential of the developed chitosan-based ternary composite as an effective biomaterial for enhanced wound healing applications.</p> Graphical Abstract <p></p>

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In vitro and in silico analysis of chitosan oligosaccharide, polyethylene glycol ternary nanocomposites for accelerated wound healing activity

  • 期刊论文

  • 发表日期：

    2026年4月3日

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  • Sweety Chellam Manuel Manohar,
  • Thaminum Ansari Abubakar,
  • Balaji Thimmarajampettai Neerazhagan,
  • Mohammad Ahmad Wadaan,
  • Nawaf D. Almoutiri,
  • Se Kwon Kim,
  • Karnan Ramachandran,
  • Vijayalakshmi Kumar,
  • Sudha Parappurath Narayanan

摘要

​The present study aimed to develop and assess a chitosan oligosaccharide (COS)-based ternary composite for enhanced wound healing using an integrated experimental and computational approach. A glutaraldehyde crosslinked COS, nano-bentonite (N-Bent), and polyethylene glycol (PEG) ternary composite in a 2:1:1 ratio was prepared and characterised using FTIR, XRD, SEM, particle size analysis, and swelling studies. The composite exhibited strong antioxidant activity in DPPH, NO, H2O2, and ABTS assays, with an antioxidant activity index of 7.47. It also demonstrated significant antibacterial and antifungal properties compared to standard drugs. Cytocompatibility of the composite with L929 fibroblast cells was confirmed using the MTT assay. The scratch wound healing test revealed the composite's ability to promote fibroblast migration with more than 92% wound closure in 36 h and significant collagen formation, indicating its potential in wound healing applications. Computational studies using molecular docking showed the composite's interaction with key wound hfaling targets such as TNF-alpha, TGF beta RI, interleukin 1 beta, and Pseudomonas aeruginosa, suggesting its dual action of antibacterial and wound-healing activity. The integrated experimental and computational findings highlight the promising potential of the developed chitosan-based ternary composite as an effective biomaterial for enhanced wound healing applications.

Graphical Abstract