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<p>Platelet-rich plasma (PRP) is a well-known biological agent with a wide spectrum of properties. Although many studies have demonstrated its osteogenic potential in vitro, the limited half-life of most growth factors in freshly isolated PRP has been a concern for clinical application. In this study, we tested whether an electrospun fibrous sheet containing lyophilized PRP (Ly-PRP) and cellulose acetate phthalate-polycaprolactone (CAP-PCL) microspheres can induce osteogenic differentiation of pre-osteoblast cells in vitro. Microspheres were developed using the water-in-oil emulsion method. Following that, Gelatin-Ly-PRP was electrospun to fabricate a fibrous non-woven scaffold, and the microspheres were immobilized onto the scaffold. The fabricated scaffolds were then subjected to FESEM, EDX, FTIR, and XRD analysis. The characterization results confirmed the successful fabrication and integration of microspheres, revealing a favourable morphology, non-toxic elemental composition, and the presence of the respective functional groups. The cytocompatibility of the fabricated scaffolds was evaluated using MG-63 human osteoblast-like cells, demonstrating enhanced cell viability in microsphere-loaded scaffolds as compared to controls. Ly-PRP activity was validated through Alamar-blue and AO/PI staining, while Masson’s Trichrome staining confirmed the presence of Ly-PRP distribution throughout the electrospun scaffold. RT-qPCR measured the expression of osteogenic genes RUNX2, ALP, BMP2, and OC, which showed elevated levels of ALP and BMP2, as well as RUNX2, indicative of the mid-phase of osteoblast cell differentiation. Results suggest that this composite scaffold is a promising candidate for regenerative medicine, particularly for bone tissue engineering, including periosteal replacement.</p>

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Gelatin-platelet rich plasma fibrous sheet loaded with cellulose acetate phthalate-polycaprolactone microspheres for periosteum bone tissue regeneration

  • 期刊论文

  • 发表日期：

    2026年3月18日

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  • U. Sakthi Priya,
  • Shanmathy Somasundaram,
  • Hanumantha Rao Balaji Raghavendran

摘要

Platelet-rich plasma (PRP) is a well-known biological agent with a wide spectrum of properties. Although many studies have demonstrated its osteogenic potential in vitro, the limited half-life of most growth factors in freshly isolated PRP has been a concern for clinical application. In this study, we tested whether an electrospun fibrous sheet containing lyophilized PRP (Ly-PRP) and cellulose acetate phthalate-polycaprolactone (CAP-PCL) microspheres can induce osteogenic differentiation of pre-osteoblast cells in vitro. Microspheres were developed using the water-in-oil emulsion method. Following that, Gelatin-Ly-PRP was electrospun to fabricate a fibrous non-woven scaffold, and the microspheres were immobilized onto the scaffold. The fabricated scaffolds were then subjected to FESEM, EDX, FTIR, and XRD analysis. The characterization results confirmed the successful fabrication and integration of microspheres, revealing a favourable morphology, non-toxic elemental composition, and the presence of the respective functional groups. The cytocompatibility of the fabricated scaffolds was evaluated using MG-63 human osteoblast-like cells, demonstrating enhanced cell viability in microsphere-loaded scaffolds as compared to controls. Ly-PRP activity was validated through Alamar-blue and AO/PI staining, while Masson’s Trichrome staining confirmed the presence of Ly-PRP distribution throughout the electrospun scaffold. RT-qPCR measured the expression of osteogenic genes RUNX2, ALP, BMP2, and OC, which showed elevated levels of ALP and BMP2, as well as RUNX2, indicative of the mid-phase of osteoblast cell differentiation. Results suggest that this composite scaffold is a promising candidate for regenerative medicine, particularly for bone tissue engineering, including periosteal replacement.