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<p>Silver nanoparticles have been prepared using the cataractous eye protein isolate that act as a reducing and stabilizing agent. The formation of the nanoparticles is confirmed by the surface plasmon resonance band at 425&#xa0;nm. The nanoparticles are spherical with radii ranging from 8&#xa0;nm to 20&#xa0;nm as revealed from TEM analyses. X-ray diffraction studies indicated that the prepared nanoparticles are crystalline in nature with a face-centered cubic arrangement. The nanoparticles were capped by the protein molecules as supported by FTIR and DLS studies. The nanoparticles exhibited good antibacterial action against <i>E. coli.</i> Further to this, nanocomposite films were prepared by mixing the prepared AgNPs (1 and 0.1% w/w of protein) with the cataractous eye protein isolate. FTIR studies revealed the presence of electrostatic and van der Waals interactions between the protein chains with nanoparticles. The film surface roughness increased with increased amounts of nanoparticles in the film matrix as showed from SEM analyses which validate the transmittance value of the films. The Young’s modulus and hardness were enhanced upon the increase in the amount of nanoparticles due to a matrix reinforcement effect (<i>P</i> &lt; 0.05). The films were non-toxic to RBCs and showed good cytocompatibility with the mouse skin fibroblast cells. The nanocomposite films showed a mild antibacterial effect against <i>E. coli.</i></p> Graphical Abstract <p></p>

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Synthesis of silver nanoparticles from the cataractous eye protein isolate for potential biomedical applications as antibacterial Nano-Hybrid films

  • 期刊论文

  • 发表日期：

    2025年4月24日

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  • Sultana Parveen,
  • Prasun Chowdhury,
  • Ragavi Rajasekaran,
  • Sreshtha Chaki,
  • Atashi Panda,
  • Atri Sen,
  • Santanu Dhara,
  • Swagata Dasgupta

摘要

Silver nanoparticles have been prepared using the cataractous eye protein isolate that act as a reducing and stabilizing agent. The formation of the nanoparticles is confirmed by the surface plasmon resonance band at 425 nm. The nanoparticles are spherical with radii ranging from 8 nm to 20 nm as revealed from TEM analyses. X-ray diffraction studies indicated that the prepared nanoparticles are crystalline in nature with a face-centered cubic arrangement. The nanoparticles were capped by the protein molecules as supported by FTIR and DLS studies. The nanoparticles exhibited good antibacterial action against E. coli. Further to this, nanocomposite films were prepared by mixing the prepared AgNPs (1 and 0.1% w/w of protein) with the cataractous eye protein isolate. FTIR studies revealed the presence of electrostatic and van der Waals interactions between the protein chains with nanoparticles. The film surface roughness increased with increased amounts of nanoparticles in the film matrix as showed from SEM analyses which validate the transmittance value of the films. The Young’s modulus and hardness were enhanced upon the increase in the amount of nanoparticles due to a matrix reinforcement effect (P < 0.05). The films were non-toxic to RBCs and showed good cytocompatibility with the mouse skin fibroblast cells. The nanocomposite films showed a mild antibacterial effect against E. coli.

Graphical Abstract