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<p>Sugarcane red stripe disease (SRSD), caused by <i>Acidovorax avenae</i> subsp. <i>avenae</i> (<i>Aaa</i>), is a bacterial disease that severely reduces sugarcane yield and quality. This study investigated the resistance effect and molecular mechanism of <i>Gluconacetobacter diazotrophicus</i> (<i>Gd</i>) against SRSD. In vitro antagonism assays showed that <i>Gd</i> significantly inhibited <i>Aaa</i>, with an inhibition zone diameter of 25&#xa0;mm. Pot experiments revealed that <i>Gd</i> inoculation reduced the disease index of the resistant variety ROC22 and susceptible variety GT58 by 28.1% and 42.94%, respectively, and significantly increased plant height, leaf length, and chlorophyll content. Integrated transcriptome and metabolome analysis indicated that <i>G. diazotrophicus</i> upregulates plasma membrane H⁺-ATPase genes (<i>PMA1</i>/<i>PMA2</i>) to activate oxidative phosphorylation (accumulating pyrophosphate for ATP-dependent defense), drives phenylpropanoid biosynthesis genes (<i>PTAL</i>, <i>CCR</i>) to synthesize lignin precursors (sinapic acid, caffeoyl quinic acid) for cell wall fortification, and activates jasmonic acid/salicylic acid defense signaling (<i>OPR</i> upregulation; JA accumulation) while balancing growth-defense resource allocation via tryptophan operon gene (<i>trpE</i>)-mediated auxin synthesis. This study provides a theoretical basis for the biological control of SRSD.</p>

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Gluconacetobacter diazotrophicus increases sugarcane resistance to red stripe disease

  • 期刊论文

  • 发表日期：

    2026年4月17日

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  • Yating Chang,
  • Ahmad Yusuf Abubakar,
  • Huiming Huang,
  • Wenzheng Li,
  • Nannan Wang,
  • Pinghua Chen

摘要

Sugarcane red stripe disease (SRSD), caused by Acidovorax avenae subsp. avenae (Aaa), is a bacterial disease that severely reduces sugarcane yield and quality. This study investigated the resistance effect and molecular mechanism of Gluconacetobacter diazotrophicus (Gd) against SRSD. In vitro antagonism assays showed that Gd significantly inhibited Aaa, with an inhibition zone diameter of 25 mm. Pot experiments revealed that Gd inoculation reduced the disease index of the resistant variety ROC22 and susceptible variety GT58 by 28.1% and 42.94%, respectively, and significantly increased plant height, leaf length, and chlorophyll content. Integrated transcriptome and metabolome analysis indicated that G. diazotrophicus upregulates plasma membrane H⁺-ATPase genes (PMA1/PMA2) to activate oxidative phosphorylation (accumulating pyrophosphate for ATP-dependent defense), drives phenylpropanoid biosynthesis genes (PTAL, CCR) to synthesize lignin precursors (sinapic acid, caffeoyl quinic acid) for cell wall fortification, and activates jasmonic acid/salicylic acid defense signaling (OPR upregulation; JA accumulation) while balancing growth-defense resource allocation via tryptophan operon gene (trpE)-mediated auxin synthesis. This study provides a theoretical basis for the biological control of SRSD.