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<p><i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (<i>Foc</i>) is the causal agent of Fusarium wilt disease, one of the most destructive pathogens affecting bananas (<i>Musa</i> spp.). <i>Foc</i>R1 and <i>Foc</i>R2 are highly virulent races that impact many commercial cultivars, some of which have been completely devastated in several countries due to their high susceptibility. Cultivars from the Cavendish subgroup have been developed through genetic engineering and are tolerant or resistant to <i>Foc</i>R1 and <i>Foc</i>R2 but remain susceptible to tropical race 4 (<i>Foc</i>TR4). In this study, we demonstrate that in vitro synthesized double-stranded RNAs (dsRNAs) targeting the <i>FKS1</i> and <i>PMT1</i> genes of <i>Foc</i>, when sprayed directly onto detached young leaves from the Manzano (AAB) and Pelipita (ABB) cultivars, mainly before but also after <i>Foc</i>R1 and/or <i>Foc</i>R2 inoculation, lead to an average reduction of over 90% in necrotic tissue and up to a 60-fold decrease in the transcript amounts of the target genes. We emphasize that, unlike HIGS, which has been shown to confer resistance against <i>Foc</i>, SIGS is a promising management strategy that does not require the generation of transgenic plants. Based on the results presented, there is no reason to suggest that this strategy will not also be successful in managing <i>Foc</i>TR4.</p>

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Spray-induced silencing of FKS1 and PMT1 genes reduces the growth and pathogenicity of Fusarium oxysporum f. sp. cubense (Foc) in bananas and plantains

  • 期刊论文

  • 发表日期：

    2026年3月19日

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  • Adrián J. Enríquez-Valencia,
  • Claudia-Anahí Pérez-Torres,
  • Emanuel Villafán,
  • Enrique Ibarra-Laclette

摘要

Fusarium oxysporum f. sp. cubense (Foc) is the causal agent of Fusarium wilt disease, one of the most destructive pathogens affecting bananas (Musa spp.). FocR1 and FocR2 are highly virulent races that impact many commercial cultivars, some of which have been completely devastated in several countries due to their high susceptibility. Cultivars from the Cavendish subgroup have been developed through genetic engineering and are tolerant or resistant to FocR1 and FocR2 but remain susceptible to tropical race 4 (FocTR4). In this study, we demonstrate that in vitro synthesized double-stranded RNAs (dsRNAs) targeting the FKS1 and PMT1 genes of Foc, when sprayed directly onto detached young leaves from the Manzano (AAB) and Pelipita (ABB) cultivars, mainly before but also after FocR1 and/or FocR2 inoculation, lead to an average reduction of over 90% in necrotic tissue and up to a 60-fold decrease in the transcript amounts of the target genes. We emphasize that, unlike HIGS, which has been shown to confer resistance against Foc, SIGS is a promising management strategy that does not require the generation of transgenic plants. Based on the results presented, there is no reason to suggest that this strategy will not also be successful in managing FocTR4.