Scholarlink

<p>Fire blight, caused by <i>Erwinia amylovora</i>, continues to spread across apple-producing regions worldwide. Genomic analyses have revealed that most global isolates belong to the “Widely Prevalent” (WP) lineage, which underwent a bottleneck during its dissemination from North America to Eurasia. Within this lineage, a distinct cluster of strains from Kazakhstan, designated the Za clade, was identified previously. In this study, we report that all Za strains share a single-base deletion in the conserved plasmid pEA29 gene <i>EAMY_3738</i>, encoding a putative ISNCY family transposase. The deletion causes a frameshift and truncation of the protein, predicted to abolish its function. The Za strains also display a distinctive CRISPR genotype, further supporting their peculiar evolutionary status. PCR testing of a set of isolates from 2023 to 2024 showed that the Za lineage is still present as a minor population in the region. This lineage may thus represent a distinct evolutionary variant.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A distinct Erwinia amylovora lineage in Kazakhstan associated with a pEA29 transposase mutation

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年12月18日

查看全文
  • Zhanna A. Berdygulova,
  • Dinara A. Naizabayeva,
  • Zhaniya M. Dosmagambet,
  • Fabio Rezzonico,
  • Theo H. M. Smits,
  • Elina R. Maltseva

摘要

Fire blight, caused by Erwinia amylovora, continues to spread across apple-producing regions worldwide. Genomic analyses have revealed that most global isolates belong to the “Widely Prevalent” (WP) lineage, which underwent a bottleneck during its dissemination from North America to Eurasia. Within this lineage, a distinct cluster of strains from Kazakhstan, designated the Za clade, was identified previously. In this study, we report that all Za strains share a single-base deletion in the conserved plasmid pEA29 gene EAMY_3738, encoding a putative ISNCY family transposase. The deletion causes a frameshift and truncation of the protein, predicted to abolish its function. The Za strains also display a distinctive CRISPR genotype, further supporting their peculiar evolutionary status. PCR testing of a set of isolates from 2023 to 2024 showed that the Za lineage is still present as a minor population in the region. This lineage may thus represent a distinct evolutionary variant.