Exploring indigenous Trichoderma spp. for biocontrol and growth promotion of Egyptian clover against Sclerotinia stem rot
摘要
Egyptian clover production in northwestern India is significantly threatened by stem rot caused by Sclerotinia sclerotiorum. This study explores the potential of thirty native Trichoderma spp. strains as biocontrol agents against this devastating pathogen. In dual culture assays, T. harzianum strains T3 and T19 exhibited the highest antifungal efficacy, with inhibition rates of 88.08% and 86.43%, respectively. Nine promising strains, belonging to two species -T. harzianum (T3, T15 and T19) and T. reesei (T12) identified through molecular techniques, demonstrated notable antagonistic activity and were further evaluated for chitinase and β-1,3-glucanase enzyme production, crucial for their biocontrol potential. Field trials revealed that the T3 (seed + soil) treatment provided the most effective disease control, reducing S. sclerotiorum severity by up to 84.19% across two seasons. This treatment also significantly enhanced both fodder biomass (809.17 q/ha in 2022-23) and seed yield (10.60 q/ha in 2022-23), outperforming chemical controls. These results demonstrate the potential of native Trichoderma strains as effective biocontrol agent, with significant antifungal activity in reducing S. sclerotiorum severity with enhanced fodder yield, offering a promising and sustainable approach to improve the productivity of Egyptian clover in the region.