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<p>Grapevine virus T (GVT) near-complete sequences were identified for the first time in Greek vineyards through the application of high-throughput sequencing in total RNA from grapevine samples. Further monitoring of GVT in Greek vineyards by RT-PCR revealed its presence in 15.32% (19/124) of the tested samples, which originated from eight mainly local cultivars situated in four viticultural areas of Greece. Complete genome and TGB1 gene (ORF2) sequence analysis revealed a high degree of genetic variability among GVT isolates, while phylogenetic analysis grouped the Greek isolates in different phylogroups, showing high genetic distances among them.</p>

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Identification and distribution of grapevine virus T in Greek vineyards

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月8日

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  • Polina Panailidou,
  • Christos Pamporis,
  • Leonidas Lotos,
  • Chrysoula-Lito Sassalou,
  • Varvara I. Maliogka

摘要

Grapevine virus T (GVT) near-complete sequences were identified for the first time in Greek vineyards through the application of high-throughput sequencing in total RNA from grapevine samples. Further monitoring of GVT in Greek vineyards by RT-PCR revealed its presence in 15.32% (19/124) of the tested samples, which originated from eight mainly local cultivars situated in four viticultural areas of Greece. Complete genome and TGB1 gene (ORF2) sequence analysis revealed a high degree of genetic variability among GVT isolates, while phylogenetic analysis grouped the Greek isolates in different phylogroups, showing high genetic distances among them.