Scholarlink

<p>Microbial elicitors play a crucial role in plant immunity, and <i>Pseudomonas putida</i> BP25, an endophytic bacterium, secretes several elicitor compounds, including siderophores, exopolysaccharides, and lipopolysaccharides, that contribute to resistance against <i>Magnaporthe oryzae</i>, the rice blast pathogen. In this study, flagellin from <i>P. putida</i> BP25 was extracted (48&#xa0;mg L⁻¹) and partially purified using ammonium sulfate precipitation, differential centrifugation, dialysis, and lyophilization. Rice seedlings of the widely cultivated Pusa Basmati 1, treated with flagellin (10 and 100&#xa0;µg mL⁻¹), exhibited characteristic reductions in shoot and root length. When three-week-old rice leaves were treated with 4 µL of flagellin (10 and 100 ng µL⁻¹), blast disease suppression ranged from 76.77 to 81.82% under prophylactic application. Flagellin-treated plants exhibited increased activity of defense-related enzymes, including peroxidase, polyphenol oxidase, and total phenols, while qPCR-based transcriptional analysis showed upregulation of key pathogenesis-related genes (<i>OsPR1.1</i>, <i>OsPR3</i>, <i>OsPDF2.2</i>), as well as <i>Oshox24</i>, <i>OsCLE</i>, and <i>OsEIN2.4</i>, whereas <i>OsACO4</i> and <i>OsACS6</i> were downregulated. These findings highlight the potential of <i>P. putida</i> BP25-derived flagellin as an immune elicitor for enhancing rice resistance against blast disease.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Endophytic Pseudomonas putida flagellin: a potent immune elicitor for the suppression of rice blast disease caused by Magnaporthe oryzae

  • 期刊论文

  • 发表日期：

    2025年4月4日

查看全文
  • Mushineni Ashajyothi,
  • Alexander Balamurugan,
  • Velmurugan Shanmugam,
  • Kuleshwar Prasad Sahu,
  • Aundy Kumar

摘要

Microbial elicitors play a crucial role in plant immunity, and Pseudomonas putida BP25, an endophytic bacterium, secretes several elicitor compounds, including siderophores, exopolysaccharides, and lipopolysaccharides, that contribute to resistance against Magnaporthe oryzae, the rice blast pathogen. In this study, flagellin from P. putida BP25 was extracted (48 mg L⁻¹) and partially purified using ammonium sulfate precipitation, differential centrifugation, dialysis, and lyophilization. Rice seedlings of the widely cultivated Pusa Basmati 1, treated with flagellin (10 and 100 µg mL⁻¹), exhibited characteristic reductions in shoot and root length. When three-week-old rice leaves were treated with 4 µL of flagellin (10 and 100 ng µL⁻¹), blast disease suppression ranged from 76.77 to 81.82% under prophylactic application. Flagellin-treated plants exhibited increased activity of defense-related enzymes, including peroxidase, polyphenol oxidase, and total phenols, while qPCR-based transcriptional analysis showed upregulation of key pathogenesis-related genes (OsPR1.1, OsPR3, OsPDF2.2), as well as Oshox24, OsCLE, and OsEIN2.4, whereas OsACO4 and OsACS6 were downregulated. These findings highlight the potential of P. putida BP25-derived flagellin as an immune elicitor for enhancing rice resistance against blast disease.