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<p>Rice seedling blight is a major concern for rice production in China. The disease is primarily caused by <i>Fusarium oxysporum</i>. To effectively control rice seedling blight, it is important to fully understand the pathogenic mechanism of this fungus. 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGR) has been shown to affect the growth and development of <i>Arabidopsis thaliana</i> and the growth and sporulation of <i>Saccharomyces cerevisiae</i>. However, HMGR has not been reported in filamentous fungi. In this study, we constructed <i>FoHmgr</i> gene deletion mutants and complement mutants of <i>F. oxysporum</i> race Fo21 to study the effect of <i>FoHmgr</i> on the growth and pathogenicity of <i>F. oxysporum</i>. We found that deletion of the <i>FoHmgr</i> gene reduced the growth rate of <i>F. oxysporum</i>, altered colony morphology, and reduced spore production by approximately 55%. It also altered its susceptibility to external stress, reduced its ability to invade infections, and decreased secretion of crude toxins. Furthermore, the disease index decreased by 71.52%, which had a significant impact on the pathogenicity of <i>F. oxysporum</i>. These findings suggest that <i>FoHmgr</i> affects the virulence of <i>F. oxysporum</i> race Fo21 by affecting its penetration ability and crude toxin secretion. In conclusion, this study shows that the <i>FoHmgr</i> gene can affect mycelial growth, sporulation, stress sensitivity, penetration ability, crude toxin secretion, and pathogenicity of <i>F. oxysporum</i>. The results of this study provide new insights into the involvement of <i>FoHmgr</i> in the pathogenesis of <i>F. oxysporum</i>.</p>

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The FoHmgr gene: a requirement for growth, conidiogenesis, stress response, crude toxin secretion, and pathogenicity in Fusarium oxysporum, causal agent of rice seedling blight

  • 期刊论文

  • 发表日期：

    2025年1月28日

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  • Yajuan Liu,
  • Songrun Yang,
  • Liang Wang,
  • Nihal Niaz,
  • Xiaofeng Xu,
  • Zhe Ni,
  • Mingxiu Yang,
  • Yuting Li,
  • Naibo Yang,
  • Junhua Zhang

摘要

Rice seedling blight is a major concern for rice production in China. The disease is primarily caused by Fusarium oxysporum. To effectively control rice seedling blight, it is important to fully understand the pathogenic mechanism of this fungus. 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGR) has been shown to affect the growth and development of Arabidopsis thaliana and the growth and sporulation of Saccharomyces cerevisiae. However, HMGR has not been reported in filamentous fungi. In this study, we constructed FoHmgr gene deletion mutants and complement mutants of F. oxysporum race Fo21 to study the effect of FoHmgr on the growth and pathogenicity of F. oxysporum. We found that deletion of the FoHmgr gene reduced the growth rate of F. oxysporum, altered colony morphology, and reduced spore production by approximately 55%. It also altered its susceptibility to external stress, reduced its ability to invade infections, and decreased secretion of crude toxins. Furthermore, the disease index decreased by 71.52%, which had a significant impact on the pathogenicity of F. oxysporum. These findings suggest that FoHmgr affects the virulence of F. oxysporum race Fo21 by affecting its penetration ability and crude toxin secretion. In conclusion, this study shows that the FoHmgr gene can affect mycelial growth, sporulation, stress sensitivity, penetration ability, crude toxin secretion, and pathogenicity of F. oxysporum. The results of this study provide new insights into the involvement of FoHmgr in the pathogenesis of F. oxysporum.