Scholarlink

<p><i>Angelica lignescens</i>, an endangered plant species endemic to the Azores Archipelago and member of the Apiaceae family, was subjected to Next Generation Sequencing (NGS) virome analysis. This analysis revealed the presence of contigs associated with a virus member of the <i>Chordovirus</i> genus. Through RT–PCR and additional Sanger sequencing, the complete genome of the virus was assembled and provisionally named angelica chordovirus (AChV). The AChV has a genome length of 8145 nt, exhibiting a similar organization to the <i>Trivirinae</i> subfamily (<i>Betaflexiviridae</i> family). Upon BLAST analysis, the highest genomic homology was uncovered with carrot chordovirus 4 (CChV4), displaying a nucleotide identity of 76%. Pairwise comparisons further emphasized this similarity, with 80% and 82% amino acid identity to the complete replicase and coat protein genes of CChV4, respectively. These values lie on the borderline of species demarcation criteria. Given that, to ensure precise classification, additional research is imperative to unravel the epidemiology and ecology of AChV. Furthermore, a more profound understanding of its impact on <i>A. lignescens</i> and its potential broader ecological implications is essential, especially concerning potential host crops.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Identification of a chordovirus hosted by Angelica lignescens

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年1月29日

查看全文
  • Sara Luna,
  • Maria Susana Lopes,
  • Eduardo Dias,
  • Dinis Pereira,
  • Artur da Câmara Machado,
  • Duarte Mendonça

摘要

Angelica lignescens, an endangered plant species endemic to the Azores Archipelago and member of the Apiaceae family, was subjected to Next Generation Sequencing (NGS) virome analysis. This analysis revealed the presence of contigs associated with a virus member of the Chordovirus genus. Through RT–PCR and additional Sanger sequencing, the complete genome of the virus was assembled and provisionally named angelica chordovirus (AChV). The AChV has a genome length of 8145 nt, exhibiting a similar organization to the Trivirinae subfamily (Betaflexiviridae family). Upon BLAST analysis, the highest genomic homology was uncovered with carrot chordovirus 4 (CChV4), displaying a nucleotide identity of 76%. Pairwise comparisons further emphasized this similarity, with 80% and 82% amino acid identity to the complete replicase and coat protein genes of CChV4, respectively. These values lie on the borderline of species demarcation criteria. Given that, to ensure precise classification, additional research is imperative to unravel the epidemiology and ecology of AChV. Furthermore, a more profound understanding of its impact on A. lignescens and its potential broader ecological implications is essential, especially concerning potential host crops.