<p>Hemostasis of incompressible trauma has been a challenge in the field of injury first aid, and these wounds are not suitable for the traditional dressings and for applying pressure to stop hemorrhage together. In this study, a Janus sponge of bioactive glass-poly (N-isopropylacrylamide) (PBG-PNIPAM) with mimic tissue structure was prepared by attaching different modified BG nanoparticles to the top and bottom layers of a polyvinyl alcohol sponge to improve hemostasis at incompressible wounds. Upon contacted with blood, the hydrophilic layer which was push into the internal tissue released Ca²⁺ and SiO₄⁴⁻ to activate the endogenous and exogenous coagulation cascade to accelerate blood coagulation, while the hydrophobic layer with excellent breathability kept in epidermis could block wound to decrease the blood loss and prevent the growth of bacteria. In the rat incompressible model, the blood loss of PBG-PNIPAM Janus sponge was much less than 2.3 times of the commercially available gelatin hemostatic sponge. In vitro results showed that the PBG-PNIPAM Janus sponge exhibited excellent clotting ability, high hemocompatibility and low cytotoxicity. This composite Janus sponge with hemostatic and antibacterial properties is expected to be applied in sharp instrument injuries.</p> Graphical Abstract <p></p>