<p>Thermal limitations of Jeffrey fluids restrict their broader industrial applications. To address this drawback, nanofluids with superior thermal properties have been introduced, though instability due to nanoparticles remains a challenge. The incorporation of motile microorganisms mitigates this issue by enhancing thermal conductivity and mass transport, stabilizing the suspension. This study investigates the magnetized bio-convective flow of a radiative Jeffrey nanofluid with motile microorganisms over a vertical permeable cone embedded in porous media, considering viscous dissipation under uniform heat and nanoparticle flux conditions. By introducing appropriate dimensionless variables, the model equations have been transitioned into non-dimensional partial differential equations and then solved using the overlapping grid-based multi-domain spectral collocation method. The numerical results for flow profiles and engineering-relevant quantities are analyzed for various flow parameters. Key findings reveal that the Deborah number, magnetic field strength, suction intensity, and porous media accelerate fluid flow, while radiative heat flux improves thermal distribution and the rate of heat conveyance. Also, intense random motion of nanoparticles and active movement of motile microbes contribute to increasing the nanoparticle mass transfer rate and density number of motile microbes.</p>