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<p>Frequent consumption of sugars by preschool-aged children disrupts the oral microbiome, culminating in dental demineralization and the subsequent development of early childhood dental caries (ECC). This study sought to identify putative salivary biomarkers for the incipient stages of ECC, thereby facilitating the early identification of children at elevated risk for caries progression. Saliva specimens were collected from children aged 24 to 71 months, categorized into ECC-affected and caries-free cohorts. These samples underwent comprehensive analysis utilizing two high-throughput methodologies: Real-time TaqMan OpenArray for microRNA (miRNA) profiling and High-Resolution Mass Spectrometry for protein profiling. Our investigations revealed the significant dysregulation of 12 miRNAs and 11 proteins in the ECC cohort. Subsequent bioinformatic analysis demonstrated an association between several miRNAs, notably miR-320, miR-378, miR-374, and miR-155, and genes implicated in inflammatory and stress-response pathways. Proteomic analysis indicated a downregulation of proteins such as PIGR, BPIFA2, BPIFA1, and MUC5B, suggesting an impaired oral immune defence. Conversely, the upregulation of HSPA8 indicated heightened oral physiological stress. While a direct correlative relationship between the dysregulated miRNAs and proteins was not definitively established, these findings collectively propose that the identified biomolecules possess potential as diagnostic risk indicators for ECC. Further validation studies are imperative to substantiate their clinical utility.</p>

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Clinical behaviour and salivary miRNAs- proteins for risk assessment of ECC

  • 期刊论文

  • 发表日期：

    2026年4月20日

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  • Monika Rathore,
  • Ankur Kumar Srivastava,
  • Ankita Srivastava,
  • Prakhar Pant,
  • Abhishek Pandeya,
  • Neerja Singh

摘要

Frequent consumption of sugars by preschool-aged children disrupts the oral microbiome, culminating in dental demineralization and the subsequent development of early childhood dental caries (ECC). This study sought to identify putative salivary biomarkers for the incipient stages of ECC, thereby facilitating the early identification of children at elevated risk for caries progression. Saliva specimens were collected from children aged 24 to 71 months, categorized into ECC-affected and caries-free cohorts. These samples underwent comprehensive analysis utilizing two high-throughput methodologies: Real-time TaqMan OpenArray for microRNA (miRNA) profiling and High-Resolution Mass Spectrometry for protein profiling. Our investigations revealed the significant dysregulation of 12 miRNAs and 11 proteins in the ECC cohort. Subsequent bioinformatic analysis demonstrated an association between several miRNAs, notably miR-320, miR-378, miR-374, and miR-155, and genes implicated in inflammatory and stress-response pathways. Proteomic analysis indicated a downregulation of proteins such as PIGR, BPIFA2, BPIFA1, and MUC5B, suggesting an impaired oral immune defence. Conversely, the upregulation of HSPA8 indicated heightened oral physiological stress. While a direct correlative relationship between the dysregulated miRNAs and proteins was not definitively established, these findings collectively propose that the identified biomolecules possess potential as diagnostic risk indicators for ECC. Further validation studies are imperative to substantiate their clinical utility.