Scholarlink

<p>As one of detection methods for the sampling physical efficiency of bioaerosol samplers, the fluorescence microsphere elution method requires multiple steps such as sampling, elution, adjusting volume, and detection, which brings about many interfering factors and low detection efficiency but wider applicable types. However, the fluorescence intensity of polystyrene fluorescent microspheres in aqueous solution is very weak, which hinders the widespread application of this method. Based on higher solubility and dispersivity, the selection of ethanol solvent achieved nearly 20 times fluorescence enhancement effect. The elution method with ethanol solvent was used to detect the physical efficiency of the same cyclonic bioaerosol sampler using 1.2&#xa0;μm fluorescent microspheres, showing good reproducibility. The sampling physical efficiency curve of fluorescence microsphere elution method shows a convex curve gradient, similar to the aerosol particle size spectrometer method, implying that the results obtained by two methods are consistent. In summary, the fluorescence microsphere elution method has low detection efficiency, but it can directly measure the concentration of particles in the sampling component without being affected by pipeline adsorption, and the range of particle size is not limited. Therefore, fluorescence microsphere elution method can be an important supplement to the aerosol particle size spectrometer method.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Research of Particle Sampling Physical Efficiency Based on Fluorescent Microsphere Elution Method

  • 期刊论文

  • 发表日期：

    2026年5月25日

查看全文
  • Ying Tian,
  • Guocheng Zhang,
  • Jiaqi Liu,
  • Xu Yan,
  • Yiting Pan,
  • Duan Feng,
  • Xinyue Lu,
  • Xingtong Qian

摘要

As one of detection methods for the sampling physical efficiency of bioaerosol samplers, the fluorescence microsphere elution method requires multiple steps such as sampling, elution, adjusting volume, and detection, which brings about many interfering factors and low detection efficiency but wider applicable types. However, the fluorescence intensity of polystyrene fluorescent microspheres in aqueous solution is very weak, which hinders the widespread application of this method. Based on higher solubility and dispersivity, the selection of ethanol solvent achieved nearly 20 times fluorescence enhancement effect. The elution method with ethanol solvent was used to detect the physical efficiency of the same cyclonic bioaerosol sampler using 1.2 μm fluorescent microspheres, showing good reproducibility. The sampling physical efficiency curve of fluorescence microsphere elution method shows a convex curve gradient, similar to the aerosol particle size spectrometer method, implying that the results obtained by two methods are consistent. In summary, the fluorescence microsphere elution method has low detection efficiency, but it can directly measure the concentration of particles in the sampling component without being affected by pipeline adsorption, and the range of particle size is not limited. Therefore, fluorescence microsphere elution method can be an important supplement to the aerosol particle size spectrometer method.