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<p>The increasing depletion of fossil fuel reserves, rising costs, and the associated greenhouse gas emissions have made searching for renewable biofuels more urgent. Anaerobic digestion (AD) presents itself as a key solution, not only for renewable energy generation but also for managing organic waste. This research evaluates the bio-methanogenic potential of poultry by-products (PBP) through AD. The Plackett–Burman design was employed to assess the significance of four key variables: pH, feathering, inoculum percentage, and inoculum type. The results revealed that substrate inoculum-to-substrate ratio and pH were the most statistically significant factors affecting methane production. Tests at pH 8, using a substrate without feathering, I/S ratio of 3, and inoculum 2, yielded the highest average methane production at 15.04 L CH₄ kg<sup>−1</sup> VS. Conversely, tests at pH 6 with feathered substrate and 50% inoculum showed the lowest yield at 3.39 L CH₄ kg<sup>−1</sup> VS. Furthermore, a model was developed to describe and improve PBP digestion efficiency. Various pre-treatments, including acid type, acid pH, substrate dilution, and added alkalinity, were employed to enhance biomethane potential of PBP. Compared to the untreated substrate, the pre-treated substrate yielded 42.51 L CH<sub>4</sub> kg<sup>−1</sup> VS, representing an increase of 182.54%. Highlighting the importance of these enhancements in improving overall methane production.</p> Graphical Abstract <p></p>

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A Combined Statistical and Pre-treatment Approach for Enhancing Methane Production from Poultry By-Products: Plackett–Burman Design and BMP Optimization

  • 期刊论文

  • 发表日期：

    2026年4月10日

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  • Ayoub Chaoui,
  • Salaheddine Farsad,
  • Aboubakr Ben Hamou,
  • Asma Amjlef,
  • Nisrine Nouj,
  • Yahia Saghir,
  • Mohamed Benafqir,
  • Mohamed Ez-zahery,
  • Noureddine El Alem

摘要

The increasing depletion of fossil fuel reserves, rising costs, and the associated greenhouse gas emissions have made searching for renewable biofuels more urgent. Anaerobic digestion (AD) presents itself as a key solution, not only for renewable energy generation but also for managing organic waste. This research evaluates the bio-methanogenic potential of poultry by-products (PBP) through AD. The Plackett–Burman design was employed to assess the significance of four key variables: pH, feathering, inoculum percentage, and inoculum type. The results revealed that substrate inoculum-to-substrate ratio and pH were the most statistically significant factors affecting methane production. Tests at pH 8, using a substrate without feathering, I/S ratio of 3, and inoculum 2, yielded the highest average methane production at 15.04 L CH₄ kg−1 VS. Conversely, tests at pH 6 with feathered substrate and 50% inoculum showed the lowest yield at 3.39 L CH₄ kg−1 VS. Furthermore, a model was developed to describe and improve PBP digestion efficiency. Various pre-treatments, including acid type, acid pH, substrate dilution, and added alkalinity, were employed to enhance biomethane potential of PBP. Compared to the untreated substrate, the pre-treated substrate yielded 42.51 L CH4 kg−1 VS, representing an increase of 182.54%. Highlighting the importance of these enhancements in improving overall methane production.

Graphical Abstract