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<p>Integration of nano biotechnology and microbiology offers a sustainable approach to wastewater treatment, enhancing pollutant degradation and resource recovery through nanoscale-biological synergies. This study investigates the potential of applying crude enzymes from the fungus <i>Phanerochaete chrysosporium</i> immobilized with TiO<sub>2</sub> nanoparticles to treat landfill leachate. Leachate samples were collected from the Pirana Solid Waste Management site in Ahmedabad, Gujarat, and thoroughly physicochemical analyzed. The TiO<sub>2</sub> nanoparticles were synthesized using a sol–gel process and characterized using a variety of techniques, including SEM, FTIR, and XRD, to confirm their shape and structural characteristics. Enzymes isolated from <i>P. chrysosporium</i> were characterized and immobilized on nanoparticles, resulting in nanoparticle-enzyme conjugations. The effectiveness of these conjugates in treating leachate was evaluated through various parameters such as pH, total dissolved solids (TDS), electrical conductivity (EC), chemical oxygen demand (COD), ammonia nitrogen (NH<sub>3</sub>–N), COD/BOD ratio, and GC–MS analysis. Following a 21-day treatment with the conjugates, the TDS decreased from 5100 ± 180 to 3400 ± 164&#xa0;ppm, and EC reduced from 10,200 ± 210 to 6800 ± 196&#xa0;µS. COD levels showed a 76% reduction, dropping from 6904 ± 250 to 1680 ± 126&#xa0;mg&#xa0;L<sup>−1</sup>, while NH<sub>3</sub>–N concentration decreased by 68%, from 1407 ± 142 to 450 ± 78&#xa0;mg L<sup>−1</sup> indicating the successful degradation of organic and inorganic pollutants. Nanoparticle based enzyme conjugates (NpChBs) offer a promising approach for the bioremediation of landfill leachate, providing an efficient and sustainable solution for mitigating environmental pollution.</p>

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To Investigate Leachate Treatment Potential of Fungus Crude Enzymes Immobilized with TiO2 Nanoparticles

  • 期刊论文

  • 发表日期：

    2025年5月23日

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  • Sachin Vaidh,
  • Dharni Parekh,
  • Dhruvesh Maiya,
  • Gajendra Singh Vishwakarma

摘要

Integration of nano biotechnology and microbiology offers a sustainable approach to wastewater treatment, enhancing pollutant degradation and resource recovery through nanoscale-biological synergies. This study investigates the potential of applying crude enzymes from the fungus Phanerochaete chrysosporium immobilized with TiO2 nanoparticles to treat landfill leachate. Leachate samples were collected from the Pirana Solid Waste Management site in Ahmedabad, Gujarat, and thoroughly physicochemical analyzed. The TiO2 nanoparticles were synthesized using a sol–gel process and characterized using a variety of techniques, including SEM, FTIR, and XRD, to confirm their shape and structural characteristics. Enzymes isolated from P. chrysosporium were characterized and immobilized on nanoparticles, resulting in nanoparticle-enzyme conjugations. The effectiveness of these conjugates in treating leachate was evaluated through various parameters such as pH, total dissolved solids (TDS), electrical conductivity (EC), chemical oxygen demand (COD), ammonia nitrogen (NH3–N), COD/BOD ratio, and GC–MS analysis. Following a 21-day treatment with the conjugates, the TDS decreased from 5100 ± 180 to 3400 ± 164 ppm, and EC reduced from 10,200 ± 210 to 6800 ± 196 µS. COD levels showed a 76% reduction, dropping from 6904 ± 250 to 1680 ± 126 mg L−1, while NH3–N concentration decreased by 68%, from 1407 ± 142 to 450 ± 78 mg L−1 indicating the successful degradation of organic and inorganic pollutants. Nanoparticle based enzyme conjugates (NpChBs) offer a promising approach for the bioremediation of landfill leachate, providing an efficient and sustainable solution for mitigating environmental pollution.