Scholarlink

<p>Precise control over reaction progression is crucial for enhancing the reliability of nanozyme-based colorimetric assays, yet conventional quenching methods often suffer from operational complexity, signal interference, or environmental contamination. To address this limitation, we synthesized photo-responsive graphene quantum dots (GQDs) derived from waste tea biomass. These GQDs exhibit oxidase-mimicking activity exclusively under light irradiation, while the catalytic activity ceases immediately in the dark, enabling real-time modulation of the reaction via simple light switching. Leveraging this light-tunable catalytic system, we discovered that D-penicillamine (DPA) acts as an effective reducing agent, quenching the photo-generated reactive oxygen species and leading to a measurable decrease in colorimetric signal. An accurate and rapid colorimetric sensing platform was developed for DPA detection, exhibiting a broad linear response range from 0.5 to 265 µM and a detection limit as low as 0.34 µM. Successful application in determining DPA content in commercial tablets confirms the potential of the proposed light-controlled nanozymatic reaction switch for practical use.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Light-Empowered Rapid Reaction Switch for Enhancing the Accuracy of Colorimetric Assay Achieved by Biomass-Derived Photonanozymes

  • 期刊论文

  • 发表日期：

    2026年6月29日

查看全文
  • Pingping Wan,
  • Hao Xu,
  • Yuping Bao,
  • Chunyan Tang,
  • Chunyan Zhang,
  • Jianxin Luo,
  • Jinsong Fan,
  • Kun Li

摘要

Precise control over reaction progression is crucial for enhancing the reliability of nanozyme-based colorimetric assays, yet conventional quenching methods often suffer from operational complexity, signal interference, or environmental contamination. To address this limitation, we synthesized photo-responsive graphene quantum dots (GQDs) derived from waste tea biomass. These GQDs exhibit oxidase-mimicking activity exclusively under light irradiation, while the catalytic activity ceases immediately in the dark, enabling real-time modulation of the reaction via simple light switching. Leveraging this light-tunable catalytic system, we discovered that D-penicillamine (DPA) acts as an effective reducing agent, quenching the photo-generated reactive oxygen species and leading to a measurable decrease in colorimetric signal. An accurate and rapid colorimetric sensing platform was developed for DPA detection, exhibiting a broad linear response range from 0.5 to 265 µM and a detection limit as low as 0.34 µM. Successful application in determining DPA content in commercial tablets confirms the potential of the proposed light-controlled nanozymatic reaction switch for practical use.