Scholarlink

<p>Developing reliable biological computing circuit devices for microRNA (miRNA) assays is critical in facilitating the early diagnosis of acute myocardial infarction (AMI), especially in distinguishing it from other cardiovascular diseases with similar symptoms. In this work, we achieved the intelligent analysis of multiple miRNAs associated with AMI and acute pulmonary embolism (APE) by integrating electrochemiluminescence (ECL) technology with DNA logic circuits. ABEI-Au@MSNs, a highly efficient ECL-emitting probe, was synthesized through the in-situ growth of ABEI-Au within mesoporous silica nanoparticles. Polymerase and endonuclease-catalyzed strand displacement amplification was combined with DNAzyme amplification technology to construct basic DNA logic gates and a cascade logic gate. The sensing performance of this strategy was evaluated in actual biological samples, validating its feasibility for the simultaneous diagnosis of multiple miRNAs. This intelligent analysis strategy provided a novel approach to early AMI diagnosis, offering promise for the accurate diagnosis of other relevant diseases.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Multiplex MicroRNA Profiling Using a Logic Nanodevice-Based Electrochemiluminescence Biosensor for the Rapid Diagnosis of Acute Myocardial Infarction

  • 期刊论文

  • 发表日期：

    2026年3月27日

查看全文
  • Yunxiang Han,
  • Runze Zhu,
  • Yao Dai,
  • Zefeng Hua,
  • Yudie Sun,
  • Kui Zhang

摘要

Developing reliable biological computing circuit devices for microRNA (miRNA) assays is critical in facilitating the early diagnosis of acute myocardial infarction (AMI), especially in distinguishing it from other cardiovascular diseases with similar symptoms. In this work, we achieved the intelligent analysis of multiple miRNAs associated with AMI and acute pulmonary embolism (APE) by integrating electrochemiluminescence (ECL) technology with DNA logic circuits. ABEI-Au@MSNs, a highly efficient ECL-emitting probe, was synthesized through the in-situ growth of ABEI-Au within mesoporous silica nanoparticles. Polymerase and endonuclease-catalyzed strand displacement amplification was combined with DNAzyme amplification technology to construct basic DNA logic gates and a cascade logic gate. The sensing performance of this strategy was evaluated in actual biological samples, validating its feasibility for the simultaneous diagnosis of multiple miRNAs. This intelligent analysis strategy provided a novel approach to early AMI diagnosis, offering promise for the accurate diagnosis of other relevant diseases.