<p>Nowadays, methods for fast and accurate detection of antibiotics have raised a huge number of concerns over their prolonged environmental persistence, posing considerable threats to environmental ecosystems and human health, leading to gastrointestinal toxicity, allergic responses, and antibiotic resistance propagation. Among the current approaches, luminescent bimetallic organic frameworks (BiM-MOFs) have attracted considerable attention in antibiotics analysis due to their adjustable coordination environment, controllable emission characteristics, and diverse structural features. In this study, the preparation strategies for BiM-MOFs were categorised as one-pot and post-synthetic modification routes. Meanwhile, each of them has been thoroughly analyzed for the advantages of achieving robust BiM-MOFs. For systematic comparison, the key representative works for antibiotics analysis using BiM-MOFs are categorized as dual-luminescent metal center, single luminescent metal center, and non-luminescent metal center, respectively, based on the sensing roles of the metal nodes. Subsequently, recent advances of each strategy for realizing the fast and accurate detection of antibiotics have been discussed and analyzed in detail. Ultimately, our aim is to provide readers with an updated roadmap for antibiotics detection by BiM-MOFs, making it easier for them to extract information, inspiring further development of bimetallic MOFs and derivatives for diverse applications.</p>