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<p>Trimethylamine N-oxide (TMAO), a small microbial metabolite, is bioactive and closely related to cardiovascular diseases (CVD) and chronic kidney diseases. Studying functions of&#xa0;TMAO calls for convenient and accurate methods to quantify TMAO. Herein, we proposed two novel chemiluminescent (CL) probes by grafting halogenated propyl ether to Schaap’s dioxetane skeleton. The detection is based on a unique oxidation reaction using TMAO as the oxidant, which uncages the luminescent scaffold to emit green luminescence. Reasonable selectivity and sensitivity were achieved on the assay with iodinated probe CLTI. An excellent linear correlation (<i>R</i><sup>2</sup> = 0.997) of CL intensity versus TMAO concentration was found at low micromolar ranges (5–50&#xa0;μM). The quantification of TMAO in partial marine fishes, as well as the isolated organs and tissues of Sequoia fishes, has been realized. This study presented probably the first CL method for TMAO determination.</p>

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Chemiluminescent Quantification of Trimethylamine Oxide in Seafoods

  • 期刊论文

  • 发表日期：

    2025年2月19日

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  • Xinxin Huang,
  • Tao Deng,
  • Qiling Wang,
  • Danfeng Peng,
  • Jialiang Guo,
  • Liying Huang,
  • Fang Liu,
  • Chun Cai

摘要

Trimethylamine N-oxide (TMAO), a small microbial metabolite, is bioactive and closely related to cardiovascular diseases (CVD) and chronic kidney diseases. Studying functions of TMAO calls for convenient and accurate methods to quantify TMAO. Herein, we proposed two novel chemiluminescent (CL) probes by grafting halogenated propyl ether to Schaap’s dioxetane skeleton. The detection is based on a unique oxidation reaction using TMAO as the oxidant, which uncages the luminescent scaffold to emit green luminescence. Reasonable selectivity and sensitivity were achieved on the assay with iodinated probe CLTI. An excellent linear correlation (R2 = 0.997) of CL intensity versus TMAO concentration was found at low micromolar ranges (5–50 μM). The quantification of TMAO in partial marine fishes, as well as the isolated organs and tissues of Sequoia fishes, has been realized. This study presented probably the first CL method for TMAO determination.