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<p>As the key factor in brain development and fat metabolism, choline is widely present in foods, especially in health care and dairy products. In this study, a simple, rapid, sensitive, and cost-effective method was proposed for choline detection based on luminol derivate (L012) doped amino silica nanoparticles (L012@SiNPs-NH<sub>2</sub>). L012@SiNPs-NH<sub>2</sub> exhibited a 47-fold increase in the electrochemiluminescence (ECL) response and much better uniformity and dispersion compared with conventional luminol-doped silica nanoparticles (Luminol@SiNPs). We thus constructed an ECL biosensor based on the L012@SiNPs-NH<sub>2</sub>/H<sub>2</sub>O<sub>2</sub> system, in which co-reactant H<sub>2</sub>O<sub>2</sub> is generated from dissolved oxygen during the process of oxidation of choline by choline oxidase. Significantly, the ECL biosensor shows a linear relationship between ECL intensity and choline concentration in the range of 1–5000&#xa0;μM, with a low limit of detection of 1&#xa0;μM. Additionally, the recovery tests from milk powders were conducted, demonstrating its practical application potential, which could be expanded to other oxidase-related analytes detection. It greatly broadened the application of L012 based nano-emitters in biological, food, and clinical analysis.</p>

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Sensitive and Rapid Electrochemiluminescent Detection of Choline Based on Luminol Derivate-Doped Silica Nanoparticles

  • 期刊论文

  • 发表日期：

    2025年1月7日

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  • Xinyu Li,
  • Guangming Li,
  • Shanshan Wu,
  • Xuewei Cao,
  • Daxi Sun,
  • Song Li,
  • Lizhen Chen,
  • Jia Zhang,
  • Hang Gao,
  • Pengfei Wei,
  • Ningning Wang

摘要

As the key factor in brain development and fat metabolism, choline is widely present in foods, especially in health care and dairy products. In this study, a simple, rapid, sensitive, and cost-effective method was proposed for choline detection based on luminol derivate (L012) doped amino silica nanoparticles (L012@SiNPs-NH2). L012@SiNPs-NH2 exhibited a 47-fold increase in the electrochemiluminescence (ECL) response and much better uniformity and dispersion compared with conventional luminol-doped silica nanoparticles (Luminol@SiNPs). We thus constructed an ECL biosensor based on the L012@SiNPs-NH2/H2O2 system, in which co-reactant H2O2 is generated from dissolved oxygen during the process of oxidation of choline by choline oxidase. Significantly, the ECL biosensor shows a linear relationship between ECL intensity and choline concentration in the range of 1–5000 μM, with a low limit of detection of 1 μM. Additionally, the recovery tests from milk powders were conducted, demonstrating its practical application potential, which could be expanded to other oxidase-related analytes detection. It greatly broadened the application of L012 based nano-emitters in biological, food, and clinical analysis.