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<p>Tea (<i>Camellia sinensis</i> (L.) O. Kuntze) ranks second in global consumption, after water, as a non-alcoholic beverage. Nearly every continent consumes it as part of its culture, traditions, and daily routines. Despite being the world’s second-largest tea producer after China, India is affected by pest and disease outbreaks. During June to August 2024 and 2025, several lesions and brown spots on tea leaves were observed in three tea gardens in the Dooars and Darjeeling regions of India. In total, three symptomatic disease samples with isolate codes PHGR01, JLAG02, and NGRF03 were collected, one each from Phuguri, Jaldhaka Altadanga, and Nagri Farm tea gardens, respectively. The fungal pathogens isolated from symptomatic leaf samples exhibited the cultural and morphological features characteristic of <i>Muyocopron</i>, which is occasionally found on the tea plant in India. The identity of the pathogen was further confirmed by pathogenicity tests and multi-locus (ITS, <i>tef-1 α</i>, and LSU) phylogenetic analysis. All three isolates showed pathogenicity on tea leaves, with lesion sizes ranging from 8.6&#xa0;mm to 13.6&#xa0;mm, and produced cell wall-degrading enzymes, including amylase, cellulase, pectinase, and protease, confirming <i>Muyocopron</i> as the causal agent of brown leaf spot disease on tea plants. The sequences of all the three isolates were identical, revealed the highest similarity (&gt; 99.9%) with the type isolate of <i>Muyocopron laterale</i> CBS 141029 in blast analysis, and clustered with type isolate with high bootstrap support (100%). This represents the first report of <i>Muyocopron laterale</i> associated with tea plants in North Bengal, India, necessitating inclusion in the country’s tea disease checklist. The findings of this study will strengthen our knowledge and help to manage brown leaf spot disease of tea.</p>

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Pathological and molecular characterization identified Muyocopron laterale as a new pathogen causing brown leaf spot disease of tea in India

  • 期刊论文

  • 发表日期：

    2026年6月29日

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  • Harshit Kumar Sharma,
  • Pranay Raj,
  • Sajeewa S. N. Maharachchikumbura,
  • Abhay Kumar Pandey

摘要

Tea (Camellia sinensis (L.) O. Kuntze) ranks second in global consumption, after water, as a non-alcoholic beverage. Nearly every continent consumes it as part of its culture, traditions, and daily routines. Despite being the world’s second-largest tea producer after China, India is affected by pest and disease outbreaks. During June to August 2024 and 2025, several lesions and brown spots on tea leaves were observed in three tea gardens in the Dooars and Darjeeling regions of India. In total, three symptomatic disease samples with isolate codes PHGR01, JLAG02, and NGRF03 were collected, one each from Phuguri, Jaldhaka Altadanga, and Nagri Farm tea gardens, respectively. The fungal pathogens isolated from symptomatic leaf samples exhibited the cultural and morphological features characteristic of Muyocopron, which is occasionally found on the tea plant in India. The identity of the pathogen was further confirmed by pathogenicity tests and multi-locus (ITS, tef-1 α, and LSU) phylogenetic analysis. All three isolates showed pathogenicity on tea leaves, with lesion sizes ranging from 8.6 mm to 13.6 mm, and produced cell wall-degrading enzymes, including amylase, cellulase, pectinase, and protease, confirming Muyocopron as the causal agent of brown leaf spot disease on tea plants. The sequences of all the three isolates were identical, revealed the highest similarity (> 99.9%) with the type isolate of Muyocopron laterale CBS 141029 in blast analysis, and clustered with type isolate with high bootstrap support (100%). This represents the first report of Muyocopron laterale associated with tea plants in North Bengal, India, necessitating inclusion in the country’s tea disease checklist. The findings of this study will strengthen our knowledge and help to manage brown leaf spot disease of tea.